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anti-AMPH antibody :: Goat AMPH / Amphiphysin Polyclonal Antibody

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Catalog # MBS242211
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

AMPH / Amphiphysin, Polyclonal Antibody

Also Known As

Goat Polyclonal to Human AMPH / Amphiphysin

Product Synonym Names
Anti-AMPH / Amphiphysin Antibody (Internal) IHC-plus; AMPH1; Amphiphysin; Amphiphysin I; Human AMPH; Amphiphysin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
695
OMIM
600418
3D Structure
ModBase 3D Structure for P49418
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat
Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
Specificity
Human AMPH / Amphiphysin. This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide C-KRKDESRISKAE from an internal region of human AMPH / Amphiphysin (NP_001626.1; NP_647477.1).
Immunogen Type
Synthetic peptide
Immunogen
AMPH / Amphiphysin antibody was raised against synthetic peptide C-KRKDESRISKAE from an internal region of human AMPH / Amphiphysin (NP_001626.1; NP_647477.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%); Xenopus (92%); Opossum, Chicken, Lizard (83%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-AMPH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AMPH antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-AMPH antibody
ELISA (1:16000), IHC-P (2.5 ug/ml), WB (0.1 - 0.5 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-AMPH antibody
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-AMPH antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-AMPH antibody
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-AMPH antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AMPH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
71,929 Da
NCBI Official Full Name
amphiphysin isoform 1
NCBI Official Synonym Full Names
amphiphysin
NCBI Official Symbol
NCBI Official Synonym Symbols
AMPH1
  [Similar Products]
NCBI Protein Information
amphiphysin; amphiphysin I; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
UniProt Protein Name
Amphiphysin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AMPH1  [Similar Products]
UniProt Entry Name
AMPH_HUMAN
NCBI Summary for AMPH
This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]
UniProt Comments for AMPH
amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: synaptic vesicle; synaptic vesicle membrane; cell junction; actin cytoskeleton

Molecular Function: protein binding; phospholipid binding

Biological Process: synaptic transmission; synaptic vesicle endocytosis; endocytosis; learning
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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