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anti-Snca antibody :: Rabbit ALPHA SYNUCLEIN Polyclonal Antibody

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Catalog # MBS222961
Unit / Price
  0.1 mL  /  $725 +1 FREE 8GB USB
anti-Snca antibody
Product Name

ALPHA SYNUCLEIN (Snca), Polyclonal Antibody

Also Known As

RABBIT ANTI RAT ALPHA SYNUCLEIN (pSer129)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
140
3D Structure
ModBase 3D Structure for P37377
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence)
Specificity
This item specifically recognises rat alpha synuclein when phosphorylated at Ser129. Alpha synuclein is a 140 amino acid, 15kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by G-protein-coupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.
Form/Format
Purified
Purified IgG - liquid
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
0.01% Bovine Serum Albumin
Immunogen
Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein.
Buffer Solution
Antiserum Preparation
Antisera to Alpha Synulein (pSer129) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-Snca antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Snca antibody
MBS222961 specifically recognises rat alpha synuclein when phosphorylated at Ser129. Alpha synuclein is a 140 amino acid, 15kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, Ser129 in particular can be phosphorylated by G-protein-coupled receptor kinases. Studies suggest that this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of Ser129 seen in Lewy bodies.
Applications Tested/Suitable for anti-Snca antibody
Western Blot (WB)*
Application Notes for anti-Snca antibody
Western Blot: This item detects a band of approximately 15kDa in rat cortex lysate.
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
NCBI/Uniprot data below describe general gene information for Snca. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
alpha-synuclein
NCBI Official Synonym Full Names
synuclein, alpha (non A4 component of amyloid precursor)
NCBI Official Symbol
NCBI Protein Information
alpha-synuclein
UniProt Protein Name
Alpha-synuclein
Protein Family
UniProt Gene Name
UniProt Entry Name
SYUA_RAT
NCBI Summary for Snca
may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD, Feb 2006]
UniProt Comments for Snca
SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Cellular Component: Golgi apparatus; nuclear outer membrane; cytoplasmic vesicle membrane; mitochondrion; rough endoplasmic reticulum; intracellular membrane-bound organelle; cell; fibril; terminal button; inclusion body; cell cortex; cytosol; actin cytoskeleton; synaptic vesicle; platelet alpha granule membrane; cytoskeleton; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; synapse; nerve terminal; nucleus

Molecular Function: identical protein binding; histone binding; zinc ion binding; kinesin binding; ferrous iron binding; magnesium ion binding; calcium ion binding; oxidoreductase activity; Hsp70 protein binding; phospholipase binding; enzyme binding; phospholipid binding; fatty acid binding; alpha-tubulin binding; protein domain specific binding; microtubule binding; caspase inhibitor activity; beta-tubulin binding; phosphoprotein binding; protein N-terminus binding; arachidonic acid binding; dynein binding; protein binding; copper ion binding; tau protein binding

Biological Process: regulation of acyl-CoA biosynthetic process; adult locomotory behavior; regulation of neurotransmitter secretion; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; positive regulation of neurotransmitter secretion; dopamine metabolic process; calcium ion homeostasis; response to magnesium ion; regulation of neuron apoptosis; synapse organization and biogenesis; negative regulation of neuron apoptosis; response to drug; negative regulation of dopamine metabolic process; synaptic vesicle endocytosis; negative regulation of transporter activity; negative regulation of apoptosis; regulation of neuronal synaptic plasticity; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; negative regulation of serotonin uptake; mitochondrial membrane organization and biogenesis; negative regulation of norepinephrine uptake; microglial cell activation; negative regulation of monooxygenase activity; negative regulation of caspase activity; fatty acid metabolic process; regulation of dopamine secretion; positive regulation of synaptic transmission; negative regulation of histone acetylation; negative regulation of dopamine uptake; synaptic transmission; negative regulation of exocytosis; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; response to iron(II) ion; positive regulation of receptor recycling; aging; caspase activation; neutral lipid metabolic process; protein destabilization; regulation of glutamate secretion; regulation of macrophage activation; negative regulation of microtubule polymerization; synaptic vesicle transport; response to cocaine; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; regulation of locomotion; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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