• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-IL12A antibody :: Rabbit IL-12A Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS8222538
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

IL-12A (IL12A), Polyclonal Antibody

Popular Item
Also Known As

Anti-IL-12A Antibody

Product Synonym Names
NKSF1; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1
Antibody/Peptide Pairs
IL-12A peptide (MBS8217269) is used for blocking the activity of IL-12A antibody (MBS8222538)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
253
OMIM
161560
3D Structure
ModBase 3D Structure for P29459
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Bovine, Dog, Porcine, Sheep
Specificity
Recognizes endogenous levels of IL-12A protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-12A. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-IL12A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IL12A antibody
Rabbit polyclonal antibody to IL-12A
Applications Tested/Suitable for anti-IL12A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IL12A antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200)

Western Blot (WB) of anti-IL12A antibody
Western blot analysis of IL-12A expression in MDA-MB231 (A) whole cell lysates.
anti-IL12A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-IL12A antibody
Immunohistochemical analysis of IL-12A staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-IL12A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for IL12A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,874 Da
NCBI Official Full Name
interleukin-12 subunit alpha
NCBI Official Synonym Full Names
interleukin 12A
NCBI Official Symbol
IL12A  [Similar Products]
NCBI Official Synonym Symbols
P35; CLMF; NFSK; NKSF1; IL-12A
  [Similar Products]
NCBI Protein Information
interleukin-12 subunit alpha
UniProt Protein Name
Interleukin-12 subunit alpha
UniProt Synonym Protein Names
Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1
Protein Family
UniProt Gene Name
IL12A  [Similar Products]
UniProt Synonym Gene Names
NKSF1; IL-12A; CLMF p35; NKSF1  [Similar Products]
UniProt Entry Name
IL12A_HUMAN
NCBI Summary for IL12A
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
UniProt Comments for IL12A
IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: extracellular space; interleukin-12 complex; cytoplasm

Molecular Function: protein binding; interleukin-27 binding; growth factor activity; interleukin-12 beta subunit binding; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding

Biological Process: cell migration; positive regulation of NK T cell activation; positive regulation of cell adhesion; negative regulation of smooth muscle cell proliferation; positive regulation of T cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of natural killer cell mediated cytotoxicity; defense response to protozoan; positive regulation of natural killer cell activation; response to UV-B; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of mononuclear cell proliferation; positive regulation of T cell differentiation; positive regulation of lymphocyte proliferation; immune response; positive regulation of T cell proliferation; cell cycle arrest
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000