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anti-APG5 antibody :: Rabbit APG5 / ATG5 Polyclonal Antibody

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Catalog # MBS241734
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

APG5 / ATG5, Polyclonal Antibody

Also Known As

Rabbit Polyclonal to Human APG5 / ATG5

Product Synonym Names
Anti-APG5 / ATG5 Antibody IHC-plus; ATG5; APG5; APG5-LIKE; APG5L; Apoptosis specific protein; Apoptosis-specific protein; Autophagy related 5; HAPG5; ASP; Autophagy protein 5; Human APG5; ATG5
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
275
OMIM
604261
3D Structure
ModBase 3D Structure for Q9H1Y0
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Bovine, Human, Mouse, Pufferfish, Pig, Rat, Zebrafish
Specificity
A portion of amino acid 1-50 of human APG5L
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.2% gelatin, 0.05% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human ATG5.
Immunogen Type
Synthetic peptide
Immunogen
APG5 / ATG5 antibody was raised against synthetic peptide from human ATG5.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Other Notes
Small volumes of anti-APG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APG5 antibody
ATG5 is required for the process of autophagy, an intracellular process for bulk degradation of cytoplasmic components. ATG5 conjugates to ATG12 and associates with isolation membranes to form autophagosomes. The autophagosomes then enclose cytoplasmic components and deliver them to lysosomes/vacuoles to be degraded. ATG5 has been shown to be involved in apoptosis with expression late in the apoptotic process downstream of caspase activity.
Applications Tested/Suitable for anti-APG5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-APG5 antibody
IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-APG5 antibody
Anti-ATG5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-APG5 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-APG5 antibody
Western blot of human APGL5 in T98G cell lysate in the 1) absence, 2) presence of immunizing peptide using antibody at 1 ug/ml.
anti-APG5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for APG5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,931 Da
NCBI Official Full Name
autophagy protein 5 isoform a
NCBI Official Synonym Full Names
autophagy related 5
NCBI Official Symbol
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
  [Similar Products]
NCBI Protein Information
autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
UniProt Protein Name
Autophagy protein 5
UniProt Synonym Protein Names
APG5-like; Apoptosis-specific protein
UniProt Gene Name
UniProt Synonym Gene Names
APG5L; ASP  [Similar Products]
UniProt Entry Name
ATG5_HUMAN
UniProt Comments for APG5
ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Apoptosis; Autophagy

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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