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anti-14-3-3 antibody :: Sheep 14-3-3 Polyclonal Antibody

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Catalog # MBS221344
Unit / Price
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  0.1 mg  /  $465 +1 FREE 8GB USB
Testing Data #1
Product Name

14-3-3, Polyclonal Antibody

Full Product Name

SHEEP ANTI 14-3-3 (pSer185)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Bovine, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat
Specificity
This item recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.Peptide sequence: EILNSPEKAC
Antiserum Preparation
Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Sheep
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from the date of despatch.
Other Notes
Small volumes of anti-14-3-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-14-3-3 antibody
MBS221344 recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
Applications Tested/Suitable for anti-14-3-3 antibody
Western Blot (WB)*
Application Notes for anti-14-3-3 antibody
Western Blot: This item detects a band of approximately 30kDa.
Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/300; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Testing Data #1 of anti-14-3-3 antibody
Western blot with Sheep anti-14-3-3 (pSER185) antibody (MBS221344)
Lane 1, 2ug recombinant 14-3-3 beta
Lane 2, 2ug recombinant 14-3-3 zeta
Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185
The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated
anti-14-3-3 antibody Testing Data #1 image
NCBI/Uniprot data below describe general gene information for 14-3-3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Secondary Accession #
Molecular Weight
18,000 Da
NCBI Official Full Name
14-3-3 protein gamma
UniProt Protein Name
14-3-3 protein gamma
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1; KCIP-1
Protein Family
UniProt Gene Name
YWHAG  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433G_SHEEP
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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