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anti-DNAJC2 antibody :: Rabbit DNAJC2 Polyclonal Antibody

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Catalog # MBS9213732
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Western Blot (WB)
Product Name

DNAJC2, Polyclonal Antibody

Popular Item
Also Known As

DNAJC2 Antibody (Center)

Product Synonym Names
DnaJ homolog subfamily C member 2; M-phase phosphoprotein 11; Zuotin-related factor 1; DnaJ homolog subfamily C member 2; N-terminally processed; DNAJC2; MPHOSPH11; MPP11; ZRF1
Antibody/Peptide Pairs
DNAJC2 peptide (MBS9228459) is used for blocking the activity of DNAJC2 antibody (MBS9213732)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
226-255
Immunogen Sequence Length
255
OMIM
605502
3D Structure
ModBase 3D Structure for Q99543
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse (Predicted Reactivity: Bovine, Monkey)
Specificity
This DNAJC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human DNAJC2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Primary Accession
Q99543
Other Accession
Q4R8H2, Q1RMH9
Precautions
DNAJC2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Cellular Location
Nucleus. Cytoplasm, cytosol.
Tissue Location
Widely expressed.
Clone Name
RB24170
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-DNAJC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DNAJC2 antibody
This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6.

Function:
Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
Product Categories/Family for anti-DNAJC2 antibody
Applications Tested/Suitable for anti-DNAJC2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-DNAJC2 antibody
WB~~1:1000

Western Blot (WB) of anti-DNAJC2 antibody
Western blot analysis of DNAJC2 Antibody (Center) in mouse Neuro-2a cell line lysates (35ug/lane). DNAJC2 (arrow) was detected using the purified Pab.
anti-DNAJC2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DNAJC2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human, mouse (Predicted Reactivity: Bovine, Monkey)NP_055192.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
71996
NCBI Official Full Name
dnaJ homolog subfamily C member 2 isoform 2
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily C, member 2
NCBI Official Symbol
DNAJC2  [Similar Products]
NCBI Official Synonym Symbols
ZRF1; ZUO1; MPP11; MPHOSPH11
  [Similar Products]
NCBI Protein Information
dnaJ homolog subfamily C member 2
UniProt Protein Name
DnaJ homolog subfamily C member 2
UniProt Synonym Protein Names
M-phase phosphoprotein 11; Zuotin-related factor 1
Protein Family
UniProt Gene Name
DNAJC2  [Similar Products]
UniProt Synonym Gene Names
MPHOSPH11; MPP11; ZRF1  [Similar Products]
UniProt Entry Name
DNJC2_HUMAN
NCBI Summary for DNAJC2
This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for DNAJC2
DNAJC2: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; DNA replication; Chaperone

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: nuclear membrane; cytoplasm; nucleus; cytosol

Molecular Function: ubiquitin binding; DNA binding; histone binding; chromatin binding; Hsp70 protein binding

Biological Process: 'de novo' cotranslational protein folding; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; chromatin modification; negative regulation of cell growth; DNA replication
Product References and Citations for anti-DNAJC2 antibody
Hatzold, J., et al. PLoS Biol. 6 (4), E84 (2008) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Otto, H., et al. Proc. Natl. Acad. Sci. U.S.A. 102(29):10064-10069(2005)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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