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anti-RELA antibody :: Rabbit NF-kappaB p65 Polyclonal Antibody

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Catalog # MBS820889
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

NF-kappaB p65 (RELA), Polyclonal Antibody

Popular Item
Also Known As

Anti-NF-kappaB p65 Antibody

Product Synonym Names
NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
548
OMIM
164014
3D Structure
ModBase 3D Structure for Q04206
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Monkey, Pig
Specificity
Recognizes endogenous levels of NF-kappaB p65 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-RELA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RELA antibody
Rabbit polyclonal antibody to NF-kappaB p65
Applications Tested/Suitable for anti-RELA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-RELA antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-RELA antibody
anti-RELA antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RELA antibody
Immunohistochemical analysis of NF-kappaB p65 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-RELA antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for RELA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,910 Da
NCBI Official Full Name
transcription factor p65 isoform 2
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog A
NCBI Official Symbol
NCBI Official Synonym Symbols
p65; NFKB3
  [Similar Products]
NCBI Protein Information
transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
UniProt Protein Name
Transcription factor p65
UniProt Synonym Protein Names
Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NFKB3  [Similar Products]
UniProt Entry Name
TF65_HUMAN
NCBI Summary for RELA
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for RELA
NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; identical protein binding; protein binding; transcription activator binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor binding; protein kinase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to cAMP; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; response to morphine; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; response to insulin stimulus; toll-like receptor 5 signaling pathway; response to organic substance; hair follicle development; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; aging; response to drug; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; acetaldehyde metabolic process; liver development; response to amino acid stimulus; toll-like receptor 2 signaling pathway; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; organ morphogenesis; positive regulation of interleukin-12 biosynthetic process; positive regulation of chondrocyte differentiation; response to mechanical stimulus; response to muramyl dipeptide; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of protein catabolic process; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; response to cobalamin; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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