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anti-ABHD12 antibody :: Rabbit ABHD12 Polyclonal Antibody

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Catalog # MBS9200845
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

ABHD12, Polyclonal Antibody

Also Known As

ABHD12 Antibody (N-term)

Product Synonym Names
Monoacylglycerol lipase ABHD12; 2-arachidonoylglycerol hydrolase; Abhydrolase domain-containing protein 12; ABHD12; C20orf22
Antibody/Peptide Pairs
ABHD12 peptide (MBS9227914) is used for blocking the activity of ABHD12 antibody (MBS9200845)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
40-66
Immunogen Sequence Length
398
OMIM
612674
3D Structure
ModBase 3D Structure for Q8N2K0
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Specificity
This ABHD12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-66 amino acids from the N-terminal region of human ABHD12.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ABHD12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ABHD12 antibody
ABHD12 has 2-arachidonoylglycerol hydrolase activity (By similarity). It may be a regulator of endocannabinoid signaling pathways (By similarity).
Applications Tested/Suitable for anti-ABHD12 antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-ABHD12 antibody
WB~~1:1000

Western Blot (WB) of anti-ABHD12 antibody
Western blot analysis of lysate from human brain tissue lysate, using ABHD12 Antibody (N-term). MBS9200845 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
anti-ABHD12 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ABHD12 antibody
Formalin-fixed and paraffin-embedded human testis tissue reacted with ABHD12 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-ABHD12 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-ABHD12 antibody
ABHD12 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ABHD12 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for ABHD12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)NP_056415.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45097
NCBI Official Full Name
monoacylglycerol lipase ABHD12 isoform a
NCBI Official Synonym Full Names
abhydrolase domain containing 12
NCBI Official Symbol
ABHD12  [Similar Products]
NCBI Official Synonym Symbols
PHARC; ABHD12A; BEM46L2; C20orf22; dJ965G21.2
  [Similar Products]
NCBI Protein Information
monoacylglycerol lipase ABHD12
UniProt Protein Name
Monoacylglycerol lipase ABHD12
UniProt Synonym Protein Names
2-arachidonoylglycerol hydrolaseCurated; Abhydrolase domain-containing protein 12Curated
Protein Family
UniProt Gene Name
ABHD12  [Similar Products]
UniProt Entry Name
ABD12_HUMAN
NCBI Summary for ABHD12
This gene encodes an enzyme that catalyzes the hydrolysis of 2-arachidonoyl glycerol (2-AG), the main endocannabinoid lipid transmitter that acts on cannabinoid receptors, CB1 and CB2. The endocannabinoid system is involved in a wide range of physiological processes, including neurotransmission, mood, appetite, pain appreciation, addiction behavior, and inflammation. Mutations in this gene are associated with the neurodegenerative disease, PHARC (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract), resulting from an inborn error of endocannabinoid metabolism. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jan 2011]
UniProt Comments for ABHD12
ABHD12: Has 2-arachidonoylglycerol hydrolase activity. May be a regulator of endocannabinoid signaling pathways. Defects in ABHD12 are the cause of polyneuropathy hearing loss ataxia retinitis pigmentosa and cataract (PHARC). PHARC is a slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses. Belongs to the serine esterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Membrane protein, integral; EC 3.1.1.23

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: integral to membrane

Molecular Function: acylglycerol lipase activity

Biological Process: phosphatidylserine catabolic process; adult walking behavior; acylglycerol catabolic process

Disease: Polyneuropathy, Hearing Loss, Ataxia, Retinitis Pigmentosa, And Cataract
Product References and Citations for anti-ABHD12 antibody
Bechtel S., et.al., BMC Genomics 8:399-399(2007).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-ABHD12 antibody
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