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anti-B4GALT1 antibody :: Rabbit B4GALT1 Polyclonal Antibody

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Catalog # MBS8242969
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

B4GALT1, Polyclonal Antibody

Popular Item
Also Known As

Anti-B4GALT1 Antibody

Product Synonym Names
GGTB2; Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Antibody/Peptide Pairs
B4GALT1 peptide (MBS8243645) is used for blocking the activity of B4GALT1 antibody (MBS8242969)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
398
OMIM
137060
3D Structure
ModBase 3D Structure for P15291
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Monkey
Specificity
Recognizes endogenous levels of B4GALT1 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human B4GALT1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-B4GALT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-B4GALT1 antibody
Rabbit polyclonal antibody to B4GALT1
Applications Tested/Suitable for anti-B4GALT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-B4GALT1 antibody
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)

Western Blot (WB) of anti-B4GALT1 antibody
Western blot analysis of B4GALT1 expression in HL60 (A), HEK293T (B), Jurkat (C) whole cell lysates.
anti-B4GALT1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-B4GALT1 antibody
Immunohistochemical analysis of B4GALT1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-B4GALT1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for B4GALT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,538 Da
NCBI Official Full Name
Beta-1,4-galactosyltransferase 1
NCBI Official Synonym Full Names
beta-1,4-galactosyltransferase 1
NCBI Official Symbol
B4GALT1  [Similar Products]
NCBI Official Synonym Symbols
GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1
  [Similar Products]
NCBI Protein Information
beta-1,4-galactosyltransferase 1
UniProt Protein Name
Beta-1,4-galactosyltransferase 1
UniProt Synonym Protein Names
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
UniProt Gene Name
B4GALT1  [Similar Products]
UniProt Synonym Gene Names
GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1  [Similar Products]
UniProt Entry Name
B4GT1_HUMAN
NCBI Summary for B4GALT1
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq, Jul 2008]
UniProt Comments for B4GALT1
B4GALT1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase 7 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 2.4.1.38; Cell adhesion; EC 2.4.1.90; Cell development/differentiation; EC 2.4.1.22; Glycan Metabolism - keratan sulfate biosynthesis; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Carbohydrate Metabolism - galactose; Motility/polarity/chemotaxis; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; Cell surface; Transferase

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: basolateral plasma membrane; brush border membrane; desmosome; external side of plasma membrane; extracellular space; filopodium; glycocalyx; Golgi apparatus; Golgi membrane; Golgi trans cisterna; integral to membrane; membrane; plasma membrane

Molecular Function: alpha-tubulin binding; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; beta-tubulin binding; cytoskeletal protein binding; galactosyltransferase activity; lactose synthase activity; manganese ion binding; N-acetyllactosamine synthase activity; protein homodimerization activity; protein kinase binding; UDP-galactosyltransferase activity

Biological Process: acute inflammatory response; angiogenesis involved in wound healing; binding of sperm to zona pellucida; branching morphogenesis of a tube; carbohydrate metabolic process; cell adhesion; cellular protein metabolic process; development of secondary sexual characteristics; epithelial cell development; extracellular matrix organization and biogenesis; galactose metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; keratan sulfate metabolic process; lactose biosynthetic process; leukocyte migration; mammary gland development; multicellular organism reproduction; negative regulation of cell proliferation; Notch signaling pathway; oligosaccharide biosynthetic process; penetration of zona pellucida; positive regulation of apoptosis involved in mammary gland involution; positive regulation of epithelial cell proliferation involved in wound healing; post-translational protein modification; protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine; regulation of acrosome reaction; regulation of cell motility; single fertilization

Disease: Congenital Disorder Of Glycosylation, Type Iid
Precautions
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Disclaimer
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