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anti-SLC9A3R1 antibody :: Rabbit SLC9A3R1 Polyclonal Antibody

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Catalog # MBS9210370
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

SLC9A3R1, Polyclonal Antibody

Popular Item
Full Product Name

SLC9A3R1 Antibody (Center)

Product Synonym Names
Na(+)/H(+) exchange regulatory cofactor NHE-RF1; NHERF-1; Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; SLC9A3R1; NHERF; NHERF1
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
168-197
OMIM
604990
3D Structure
ModBase 3D Structure for O14745
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
Specificity
This SLC9A3R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human SLC9A3R1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-SLC9A3R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SLC9A3R1 antibody
Function: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

Cellular Location: Cytoplasm. Apical cell membrane. Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle Cell projection, microvillus. Note=Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T- cells.

Tissue Location: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
Product Categories/Family for anti-SLC9A3R1 antibody
Applications Tested/Suitable for anti-SLC9A3R1 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-SLC9A3R1 antibody
WB~~1:1000; FC~~1:10~50

Western Blot (WB) of anti-SLC9A3R1 antibody
All lanes : Anti-SLC9A3R1 Antibody (Center) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: T47D whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 39 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-SLC9A3R1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-SLC9A3R1 antibody
SLC9A3R1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SLC9A3R1 antibody detected the SLC9A3R1 protein (arrow).
anti-SLC9A3R1 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-SLC9A3R1 antibody
SLC9A3R1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-SLC9A3R1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for SLC9A3R1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38868
NCBI Official Full Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
NCBI Official Symbol
SLC9A3R1  [Similar Products]
NCBI Official Synonym Symbols
EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
  [Similar Products]
NCBI Protein Information
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
UniProt Protein Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
UniProt Synonym Protein Names
Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1
UniProt Gene Name
SLC9A3R1  [Similar Products]
UniProt Synonym Gene Names
NHERF; NHERF1; NHERF-1; EBP50  [Similar Products]
UniProt Entry Name
NHRF1_HUMAN
NCBI Summary for SLC9A3R1
This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]
UniProt Comments for SLC9A3R1
NHERF: a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; brush border membrane; endomembrane system; lipid raft; actin cytoskeleton; ruffle; membrane; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; nucleus; vesicle; filopodium

Molecular Function: protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; protein complex binding; beta-catenin binding; dopamine receptor binding; protein complex scaffold; phosphatase binding; PDZ domain binding; receptor binding

Biological Process: bile acid secretion; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; renal sodium ion transport; negative regulation of cell proliferation; microvillus biogenesis; cellular phosphate ion homeostasis; regulation of sodium:hydrogen antiporter activity; protein complex assembly; dopamine receptor, phospholipase C activating pathway; negative regulation of sodium:hydrogen antiporter activity; regulation of protein kinase activity; actin cytoskeleton organization and biogenesis; negative regulation of cell migration; negative regulation of phosphoinositide 3-kinase cascade; dopamine receptor, adenylate cyclase activating pathway

Disease: Nephrolithiasis/osteoporosis, Hypophosphatemic, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withanti-SLC9A3R1 antibody
 Reference Product  PubMed Publications
 CFTR antibody  >1 publications with SLC9A3R1 and CFTR
Pathways associated with anti-SLC9A3R1 antibody
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