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anti-AIOLOS antibody :: Rabbit AIOLOS Polyclonal Antibody

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Catalog # MBS8303447
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Product Name

AIOLOS, Polyclonal Antibody

Full Product Name

Anti-AIOLOS Antibody

Product Synonym Names
Rabbit polyclonal antibody to AIOLOS; ZNFN1A3; Zinc finger protein Aiolos; Ikaros family zinc finger protein 3
Product Synonym Gene Name
Antibody/Peptide Pairs
AIOLOS peptide (MBS8303737) is used for blocking the activity of AIOLOS antibody (MBS8303447)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
606221
3D Structure
ModBase 3D Structure for Q9UKT9
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Bovine, Monkey
Specificity
Recognizes endogenous levels of AIOLOS protein.
Purity/Purification
Purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AIOLOS. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-AIOLOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AIOLOS antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-AIOLOS antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Wesern Blot (WB) of anti-AIOLOS antibody
Western blot analysis of AIOLOS expression in HepG2 (A), HeLa (B) whole cell lysates.
anti-AIOLOS antibody Wesern Blot (WB) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-AIOLOS antibody
Immunofluorescent analysis of AIOLOS staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-AIOLOS antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for AIOLOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,973 Da
NCBI Official Full Name
zinc finger protein Aiolos isoform 7
NCBI Official Synonym Full Names
IKAROS family zinc finger 3
NCBI Official Symbol
IKZF3  [Similar Products]
NCBI Official Synonym Symbols
AIO; AIOLOS; ZNFN1A3
  [Similar Products]
NCBI Protein Information
zinc finger protein Aiolos
UniProt Protein Name
Zinc finger protein Aiolos
UniProt Synonym Protein Names
Ikaros family zinc finger protein 3
UniProt Gene Name
IKZF3  [Similar Products]
UniProt Synonym Gene Names
ZNFN1A3  [Similar Products]
NCBI Summary for AIOLOS
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]
UniProt Comments for AIOLOS
Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-AIOLOS antibody
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