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anti-MAP1LC3B antibody :: Rabbit Cleaved LC3B Polyclonal Antibody

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Catalog # MBS9205943
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

Cleaved LC3B (MAP1LC3B), Polyclonal Antibody

Popular Item
Full Product Name

Cleaved LC3B Antibody

Product Synonym Names
Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
89-122
OMIM
609604
3D Structure
ModBase 3D Structure for Q9GZQ8
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Specificity
This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.25 (lot specific)
Antigen Type
Synthetic Peptide
Other Accession
O41515
Antigen Source
HUMAN
Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Cellular Location
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Tissue Location
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-MAP1LC3B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MAP1LC3B antibody
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Applications Tested/Suitable for anti-MAP1LC3B antibody
Immunofluorescence (IF), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes for anti-MAP1LC3B antibody
IF~~1:10~50
ICC~~1:10~50

Immunofluorescence (IF) of anti-MAP1LC3B antibody
Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9205943 cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
anti-MAP1LC3B antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-MAP1LC3B antibody
Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
anti-MAP1LC3B antibody Immunofluorescence (IF) image
Immunocytochemistry (ICC) of anti-MAP1LC3B antibody
SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
anti-MAP1LC3B antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for MAP1LC3B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14688
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3B
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol
MAP1LC3B  [Similar Products]
NCBI Official Synonym Symbols
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
  [Similar Products]
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names
Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name
MAP1LC3B  [Similar Products]
UniProt Synonym Gene Names
MAP1ALC3; MAP1A/MAP1B LC3 B  [Similar Products]
UniProt Entry Name
MLP3B_HUMAN
NCBI Summary for MAP1LC3B
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Comments for MAP1LC3B
LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.

Protein type: Vesicle; Ubiquitin-like modifier; Autophagy; Microtubule-binding

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle; axoneme; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
Product References and Citations for anti-MAP1LC3B antibody
References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4. Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].


Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Pathways associated with anti-MAP1LC3B antibody
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