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anti-RBM14 antibody :: Rabbit RBM14 Polyclonal Antibody

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Catalog # MBS9206830
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

RBM14, Polyclonal Antibody

Popular Item
Full Product Name

RBM14 Antibody (C-term)

Product Synonym Names
RNA-binding protein 14; Paraspeckle protein 2; PSP2; RNA-binding motif protein 14; RRM-containing coactivator activator/modulator; Synaptotagmin-interacting protein; SYT-interacting protein; RBM14; SIP
Antibody/Peptide Pairs
RBM14 peptide (MBS9218465) is used for blocking the activity of RBM14 antibody (MBS9206830)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
563-594
OMIM
612409
3D Structure
ModBase 3D Structure for Q96PK6
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Specificity
This RBM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 563-594 amino acids from the C-terminal region of human RBM14.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
0.5mg/ml (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.
Cellular Location
Nucleus. Nucleus, nucleolus. Note=In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles
Tissue Location
Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-RBM14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RBM14 antibody
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.
Applications Tested/Suitable for anti-RBM14 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-RBM14 antibody
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50

Western Blot (WB) of anti-RBM14 antibody
RBM14 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RBM14 antibody detected the RBM14 protein (arrow).
anti-RBM14 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RBM14 antibody
RBM14 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM14 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-RBM14 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-RBM14 antibody
RBM14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
anti-RBM14 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for RBM14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human (Predicted Reactivity: Bovine, Mouse)NP_001185766.1; NP_001185774.1; NP_001185775.1; NP_006319.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69492
NCBI Official Full Name
RNA-binding protein 14 isoform 2
NCBI Official Synonym Full Names
RNA binding motif protein 14
NCBI Official Symbol
RBM14  [Similar Products]
NCBI Official Synonym Symbols
SIP; COAA; PSP2; SYTIP1; TMEM137
  [Similar Products]
NCBI Protein Information
RNA-binding protein 14
UniProt Protein Name
RNA-binding protein 14
UniProt Synonym Protein Names
Paraspeckle protein 2; PSP2; RNA-binding motif protein 14; RRM-containing coactivator activator/modulator; Synaptotagmin-interacting protein; SYT-interacting protein
Protein Family
UniProt Gene Name
RBM14  [Similar Products]
UniProt Synonym Gene Names
SIP; PSP2; SYT-interacting protein  [Similar Products]
UniProt Entry Name
RBM14_HUMAN
NCBI Summary for RBM14
This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for RBM14
RBM14: an RNA binding protein and transcriptional regulator. Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. Two alternatively spliced isoforms have been observed. Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.

Protein type: Nucleolus; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; transcription factor complex; nucleolus; Srb-mediator complex; ribonucleoprotein complex

Molecular Function: protein binding, bridging; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; estrogen receptor signaling pathway; glucocorticoid receptor signaling pathway; response to hormone stimulus; histone deacetylation; positive regulation of transcription from RNA polymerase II promoter; DNA replication; DNA repair; DNA recombination
Product References and Citations for anti-RBM14 antibody
Li, X., et al. J. Cell. Biochem. 108(2):378-387(2009)
Brooks, Y.S., et al. J. Biol. Chem. 284(27):18033-18046(2009)
Kang, Y.K., et al. Cancer Res. 68(19):7887-7896(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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