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anti-KRT5 antibody :: Cytokeratin 5 Polyclonal Antibody

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Catalog # MBS822090
Unit / Price
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  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Cytokeratin 5 (KRT5), Polyclonal Antibody

Popular Item
Full Product Name

Anti-Cytokeratin 5 Antibody

Product Synonym Names
Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 609352
3D Structure
ModBase 3D Structure for P13647
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat, Bovine, Pig
Specificity
Recognizes endogenous levels of Cytokeratin 5 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Source
Rabbit
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 5. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-KRT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KRT5 antibody
Rabbit polyclonal antibody to Cytokeratin 5
Applications Tested/Suitable for anti-KRT5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-KRT5 antibody
WB (1/500 - 1/1000)
IH (1/100 - 1/200)
IF/ICC (1/100 - 1/500)

Western Blot (WB) of anti-KRT5 antibody
Western blot analysis of Cytokeratin 5 expression in DU145 (A), HeLa (B), MCF7 (C) whole cell lysates.
anti-KRT5 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-KRT5 antibody
Immunohistochemical analysis of Cytokeratin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-KRT5 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-KRT5 antibody
Immunofluorescent analysis of Cytokeratin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-KRT5 antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for KRT5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,378 Da
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
NCBI Official Synonym Symbols
K5; CK5; DDD; DDD1; EBS2; KRT5A
  [Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)
UniProt Protein Name
Keratin, type II cytoskeletal 5
UniProt Synonym Protein Names
58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CK-5; K5  [Similar Products]
UniProt Entry Name
K2C5_HUMAN
NCBI Summary for KRT5
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
UniProt Comments for KRT5
K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: mitochondrion; membrane; cytoplasm; keratin filament; plasma membrane; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-KRT5 antibody
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