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anti-EIF4EBP1 antibody :: Rabbit 4EBP1 Polyclonal Antibody

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Catalog # MBS8211698
Unit / Price
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  0.03 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $245 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

4EBP1 (EIF4EBP1), Polyclonal Antibody

Full Product Name

Anti-4EBP1 (pT37) Antibody

Product Synonym Names
Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Antibody/Peptide Pairs
4EBP1 peptide (MBS8233507) is used for blocking the activity of 4EBP1 antibody (MBS8211698)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602223
3D Structure
ModBase 3D Structure for Q13541
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine, Porcine
Specificity
Recognizes endogenous levels of 4EBP1 (pT37) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 4EBP1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-EIF4EBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EIF4EBP1 antibody
Rabbit polyclonal antibody to 4EBP1 (pT37)
Applications Tested/Suitable for anti-EIF4EBP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EIF4EBP1 antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200)

Western Blot (WB) of anti-EIF4EBP1 antibody
Western blot analysis of 4EBP1 (pT37) expression in HEK293T insulin-treated (A), NIH3T3 insulin-treated (B), PC12 insulin-treated (C) whole cell lysates.
anti-EIF4EBP1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-EIF4EBP1 antibody
Immunohistochemical analysis of 4EBP1 (pT37) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-EIF4EBP1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for EIF4EBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,580 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1  [Similar Products]
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UniProt Gene Name
EIF4EBP1  [Similar Products]
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I  [Similar Products]
UniProt Entry Name
4EBP1_HUMAN
NCBI Summary for EIF4EBP1
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Comments for EIF4EBP1
4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; cellular protein metabolic process; TOR signaling pathway; response to ethanol; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; lung development; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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