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anti-AAK1 antibody :: Rabbit AAK1 Polyclonal Antibody

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Catalog # MBS8248146
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

AAK1, Polyclonal Antibody

Also Known As

Anti-AAK1 Antibody

Product Synonym Names
KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1
Antibody/Peptide Pairs
AAK1 peptide (MBS8247858) is used for blocking the activity of AAK1 antibody (MBS8248146)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
961
OMIM
616405
3D Structure
ModBase 3D Structure for Q2M2I8
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Bovine
Specificity
Recognizes endogenous levels of AAK1 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AAK1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-AAK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AAK1 antibody
Rabbit polyclonal antibody to AAK1
Applications Tested/Suitable for anti-AAK1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-AAK1 antibody
WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200; Immunofluorescence/Immunocytochemistry: 1/50 - 1/100

Western Blot (WB) of anti-AAK1 antibody
Western blot analysis of AAK1 expression in HepG2 (A), COS7 (B) whole cell lysates.
anti-AAK1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AAK1 antibody
Immunohistochemical analysis of AAK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-AAK1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-AAK1 antibody
Immunofluorescent analysis of AAK1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-AAK1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for AAK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
93,578 Da
NCBI Official Full Name
AP2-associated protein kinase 1
NCBI Official Synonym Full Names
AP2 associated kinase 1
NCBI Official Symbol
NCBI Protein Information
AP2-associated protein kinase 1
UniProt Protein Name
AP2-associated protein kinase 1
UniProt Synonym Protein Names
Adaptor-associated kinase 1
UniProt Gene Name
UniProt Synonym Gene Names
KIAA1048  [Similar Products]
UniProt Entry Name
AAK1_HUMAN
NCBI Summary for AAK1
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for AAK1
AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Protein type: EC 2.7.11.1; Kinase, protein; NAK family; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: clathrin-coated vesicle; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button

Molecular Function: Notch binding; protein binding; protein serine/threonine kinase activity

Biological Process: positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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