• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-COL3A1 antibody :: Rabbit Collagen Type III Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS534144
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot
Product Name

Collagen Type III (COL3A1), Polyclonal Antibody

Full Product Name

Collagen Type III antibody

Product Synonym Names
Polyclonal Collagen Type III; Anti-Collagen Type III; Fetal collagen; autosomal dominant; Collagen type III alpha; Collagen type III alpha 1; EDS4A; Ehlers Danlos syndrome type IV
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
100070
3D Structure
ModBase 3D Structure for P02461
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Bovine/Human collagen type III
Purity/Purification
Collagen Type III antibody was purified by affinity chromatography.
Form/Format
Sterile filtered liquid in 0.125M Na2B4O7, pH 8.0, with 0.075M NaCl, 0.005M EDTA, and 0.01% NaN3.
Concentration
2.0 mg/ml (lot specific)
Bioactivity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Collagen type III antibody was raised in rabbit using collagen type III from human and bovine placenta as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-COL3A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL3A1 antibody
Affinity purified Rabbit polyclonal Collagen Type III antibody
Applications Tested/Suitable for anti-COL3A1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-COL3A1 antibody
ELISA: 1:5,000-1:10,000
IHC: 1:50-1:200
WB: 1:500-1:1,000

Western Blot of anti-COL3A1 antibody
Western Blot analysis using Collagen Type III antibody
Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III antibody at 1:1000 ON at 4 deg C. Secondary antibody: Dylight (TM) 649 Goat anti-rabbit at 1:20, 000 for 30 min at RT. Block for 30 min at RT.
anti-COL3A1 antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for COL3A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
111,907 Da[Similar Products]
NCBI Official Full Name
collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant), isoform CRA_b
NCBI Official Synonym Full Names
collagen, type III, alpha 1
NCBI Official Symbol
COL3A1  [Similar Products]
NCBI Official Synonym Symbols
EDS4A
  [Similar Products]
NCBI Protein Information
collagen alpha-1(III) chain
UniProt Protein Name
Collagen alpha-1(III) chain
Protein Family
UniProt Gene Name
COL3A1  [Similar Products]
UniProt Entry Name
CO3A1_HUMAN
NCBI Summary for COL3A1
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
UniProt Comments for COL3A1
CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Cell adhesion; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region; collagen type III

Molecular Function: integrin binding; protein binding; metal ion binding; extracellular matrix structural constituent; platelet-derived growth factor binding; SMAD binding

Biological Process: integrin-mediated signaling pathway; skin development; platelet activation; receptor-mediated endocytosis; axon guidance; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; cell-matrix adhesion; heart development; negative regulation of immune response; positive regulation of Rho protein signal transduction; extracellular matrix disassembly; collagen catabolic process; response to radiation; gut development; response to mechanical stimulus; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; fibril organization and biogenesis; cerebral cortex development; peptide cross-linking; skeletal development; aging

Disease: Ehlers-danlos Syndrome, Type Iv, Autosomal Dominant; Ehlers-danlos Syndrome, Type Iii
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000