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anti-AAK1 antibody :: Rabbit AAK1 Polyclonal Antibody

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Catalog # MBS9212426
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Western Blot (WB)
Product Name

AAK1, Polyclonal Antibody

Also Known As

AAK1 Antibody (N-term)

Product Synonym Names
AP2-associated protein kinase 1; Adaptor-associated kinase 1; AAK1; KIAA1048
Antibody/Peptide Pairs
AAK1 peptide (MBS9227326) is used for blocking the activity of AAK1 antibody (MBS9212426)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
186-216
Immunogen Sequence Length
863
3D Structure
ModBase 3D Structure for Q2M2I8
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Bovine, Pig)
Specificity
This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the N-terminal region of human AAK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-AAK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AAK1 antibody
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.
Product Categories/Family for anti-AAK1 antibody
Applications Tested/Suitable for anti-AAK1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AAK1 antibody
WB~~1:1000

Western Blot (WB) of anti-AAK1 antibody
Western blot analysis of AAK1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.
anti-AAK1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AAK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
103885
NCBI Official Full Name
AP2-associated protein kinase 1
NCBI Official Synonym Full Names
AP2 associated kinase 1
NCBI Official Symbol
NCBI Protein Information
AP2-associated protein kinase 1
UniProt Protein Name
AP2-associated protein kinase 1
UniProt Synonym Protein Names
Adaptor-associated kinase 1
UniProt Gene Name
UniProt Synonym Gene Names
KIAA1048  [Similar Products]
UniProt Entry Name
AAK1_HUMAN
NCBI Summary for AAK1
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for AAK1
AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NAK family

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: extrinsic to plasma membrane; clathrin-coated vesicle; leading edge; terminal button; coated pit

Molecular Function: protein serine/threonine kinase activity; protein binding; Notch binding; ATP binding

Biological Process: regulation of protein localization; protein stabilization; protein amino acid autophosphorylation; endocytosis; protein amino acid phosphorylation; positive regulation of Notch signaling pathway
Product References and Citations for anti-AAK1 antibody
Henderson,D.M., Mol. Biol. Cell 18 (7), 2698-2706 (2007)
Takahashi,T., Cancer Res. 66 (24), 11932-11937 (2006)
Schmid,E.M., PLoS Biol. 4 (9), E262 (2006)

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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