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anti-MYLK antibody :: Rabbit Myosin Light Chain Kinase, Smooth Muscle Polyclonal Antibody

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Catalog # MBS618405
Unit / Price
  10 Blots  /  $620 +1 FREE 8GB USB
anti-MYLK antibody
Product Name

Myosin Light Chain Kinase, Smooth Muscle (MYLK), Polyclonal Antibody

Also Known As

Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)

Product Synonym Names
Anti -Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 3; NC_000003.11 (123331143..123603149, complement). Location: 3q21
OMIM
600922
3D Structure
ModBase 3D Structure for Q15746
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Bovine, Mouse, Rabbit
Specificity
Recognizes MLCK when phosphorylated at Ser1760. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Immunogen
Synthetic phosphopeptide derived from the region of human myosin light chain kinase (MLCK) that contains serine 1760.
Positive Control
KCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short MLCK, phosphorylated in vitro by PKA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-MYLK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MYLK antibody
Smooth Muscle Myosin Light Chain Kinase (MLCK) is a multifunctional regulatory protein of smooth muscle contraction (SMC) and a key element in ligand-mediated endothelial cell gap formation and vascular permeability, motility and morphology. Smooth muscle MLCK exists in at least two isoforms, short (~150kD) and long (210kD) which are identical except for an extended amino terminus with two additional putative actin-binding motifs in the long isoform.
Product Categories/Family for anti-MYLK antibody
Applications Tested/Suitable for anti-MYLK antibody
Western Blot (WB)
Application Notes for anti-MYLK antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
NCBI/Uniprot data below describe general gene information for MYLK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
210,715 Da[Similar Products]
NCBI Official Full Name
myosin light chain kinase, smooth muscle isoform 1
NCBI Official Synonym Full Names
myosin light chain kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083; FLJ12216; DKFZp686I10125
  [Similar Products]
NCBI Protein Information
myosin light chain kinase, smooth muscle; telokin; OTTHUMP00000180642; OTTHUMP00000180643; OTTHUMP00000215974; OTTHUMP00000225212; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
UniProt Protein Name
Myosin light chain kinase, smooth muscle
UniProt Synonym Protein Names
Kinase-related protein; KRP; Telokin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
MYLK_HUMAN
NCBI Summary for MYLK
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq]
UniProt Comments for MYLK
smMLCK: Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA- dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis. Defects in MYLK are the cause of familial aortic aneurysm thoracic type 7 (AAT7). AAT7 is a disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.18; CAMK group; MLCK family

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: lamellipodium; cytoplasm; stress fiber; intercellular junction; cytosol; cleavage furrow

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; metal ion binding; actin binding; ATP binding; myosin light chain kinase activity

Biological Process: tonic smooth muscle contraction; smooth muscle contraction; muscle contraction; actin filament organization; bleb formation; positive regulation of calcium ion transport; protein amino acid phosphorylation; positive regulation of cell migration

Disease: Aortic Aneurysm, Familial Thoracic 7
Precautions
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Disclaimer
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