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anti-RPS27A antibody :: Rabbit anti-Bovine Ubiquitin Polyclonal Antibody

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Catalog # MBS560605
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anti-RPS27A antibody
Product Name

Ubiquitin (RPS27A), Polyclonal Antibody

Full Product Name

Rabbit anti-Ubiquitin (Bovine)

Product Synonym Names
Rabbit anti-Bovine Ubiquitin; anti-Bovine Ubiquitin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (55459039..55462989). Location: 2p16
OMIM
191343
3D Structure
ModBase 3D Structure for P62979
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Bovine
Form/Format
Whole Serum
Physical State: Liquid
Preservative
0.1% sodium azide
Procedure
Rabbits were immunized with purified bovine ubiquitin coupled with KLH and serum was collected.
Preparation and Storage
Store at 4 degree C.
Shelf Life: 12 months from shipment date
Other Notes
Small volumes of anti-RPS27A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RPS27A antibody
Rabbits were immunized with purified bovine ubiquitin.
Product Categories/Family for anti-RPS27A antibody
Application Notes for anti-RPS27A antibody
This polyclonal antibody reacts with ubiquitin by ELISA and western blot. Suggested starting dilution: ELISA = 1:500 Blotting = 1:150. Optimal working dilutions should be determined for your particular assay conditions.
NCBI/Uniprot data below describe general gene information for RPS27A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,965 Da[Similar Products]
NCBI Official Full Name
ubiquitin
NCBI Official Synonym Full Names
ribosomal protein S27a
NCBI Official Symbol
RPS27A  [Similar Products]
NCBI Official Synonym Symbols
UBC; S27A; CEP80; UBA80; UBCEP1; UBCEP80
  [Similar Products]
NCBI Protein Information
ubiquitin-40S ribosomal protein S27a; ubiquitin C; ubiquitin-CEP80; OTTHUMP00000200786; OTTHUMP00000200788; OTTHUMP00000200789; OTTHUMP00000201692; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80
UniProt Protein Name
Ubiquitin-40S ribosomal protein S27a
UniProt Synonym Protein Names
Ubiquitin carboxyl extension protein 80
Protein Family
UniProt Gene Name
RPS27A  [Similar Products]
UniProt Synonym Gene Names
UBA80; UBCEP1  [Similar Products]
UniProt Entry Name
RS27A_HUMAN
NCBI Summary for RPS27A
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq]
UniProt Comments for RPS27A
RPS27A: the gene (RPS27A) that encodes this protein is one of four that encode for ubiquitin: UBC, UBB, UBA52 and RPS27A. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The RPS27A gene product is cleaved into the following 2 chains: ubiquitin (amino acids 1-76) and the 40S ribosomal protein S27a (amino acids 77-156). Ubiquitin is a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. At the protein level, it is not possible to easily determine which of the four genes encoded a given ubiquitin chain.

Protein type: Ubiquitin-like modifier; Ribosomal

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: nucleoplasm; small ribosomal subunit; membrane; cytoplasm; nucleolus; plasma membrane; endosome membrane; cytosol

Molecular Function: structural constituent of ribosome; metal ion binding

Biological Process: circadian rhythm; SRP-dependent cotranslational protein targeting to membrane; I-kappaB kinase/NF-kappaB cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; translational initiation; antigen processing and presentation of exogenous peptide antigen via MHC class I; JNK cascade; viral transcription; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; Notch receptor processing; glucose metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; virus assembly; toll-like receptor 2 signaling pathway; translational elongation; carbohydrate metabolic process; mRNA catabolic process, nonsense-mediated decay; viral protein processing; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; translation; apoptosis; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; viral infectious cycle; translational termination; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; DNA repair; MyD88-dependent toll-like receptor signaling pathway; cellular protein metabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway
Precautions
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Disclaimer
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