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anti-OCLN antibody :: Rabbit Occludin Polyclonal Antibody

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Catalog # MBS621431
Unit / Price
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  0.1 mg  /  $620 +1 FREE 8GB USB
anti-OCLN antibody
Product Name

Occludin (OCLN), Polyclonal Antibody

Full Product Name

Occludin

Product Synonym Names
Anti -Occludin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 5; NC_000005.9 (68788119..68850397). Location: 5q13.1
OMIM
251290
3D Structure
ModBase 3D Structure for Q16625
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Canine, Human, Mammal, Mouse, Rat
Specificity
Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species Crossreactivity: mammalian, mouse, rat and canine
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Immunogen
Fusion protein containing the C-terminal 150aa of human occludin.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-OCLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-OCLN antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-OCLN antibody
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunofluorescence: 4-20ug/ml
Immunoprecipitation: ~5ug/IP reaction
Immunohistochemistry (FFPE): 2-5ug/ml; Protease pretreatment of sections is required prior to staining. Dilute protease to 1-2mg/ml and incubate at 37 degree C for 10 minutes. Other enzyme and HIER pretreatment methods may not be suitable for this antibody.
NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,144 Da[Similar Products]
NCBI Official Full Name
occludin isoform a
NCBI Official Synonym Full Names
occludin
NCBI Official Symbol
NCBI Official Synonym Symbols
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277
  [Similar Products]
NCBI Protein Information
occludin; OTTHUMP00000125187; OTTHUMP00000222347; tight junction protein occludin
UniProt Protein Name
Occludin
Protein Family
UniProt Gene Name
UniProt Entry Name
OCLN_HUMAN
NCBI Summary for OCLN
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq]
UniProt Comments for OCLN
occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-OCLN antibody
 Reference Product  PubMed Publications
 TJP1 antibody  >3 publications with OCLN and TJP1
Organs/Tissues associated with anti-OCLN antibody
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