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anti-COL4A5 antibody :: Rabbit Collagen Type IV Polyclonal Antibody

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Catalog # MBS534314
Unit / Price
  0.25 mL  /  $495 +1 FREE 8GB USB
anti-COL4A5 antibody
Product Name

Collagen Type IV (COL4A5), Polyclonal Antibody

Also Known As

Collagen Type IV antibody

Product Synonym Names
Polyclonal Collagen Type IV; Anti-Collagen Type IV; Canstatin; Collagen Of Basement Membrane alpha 1 Chain; Arresten
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
18
OMIM
301050
3D Structure
ModBase 3D Structure for P29400
Clonality
Polyclonal
Isotype
IgG1
Host
Rabbit
Species Reactivity
Cat, Dog, Fish, Human, Mouse, Pig, Rat
Specificity
Human collagen type IV
Form/Format
PBS, with 1% BSA and 0.1% NaN3
Biological Significance
Type-IV collagen is a type of collagen found primarily in the basal lamina. The C-terminus domain is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, type-IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Collagen type IV antibody was raised in rabbit using human placenta type IV collagen as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-COL4A5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL4A5 antibody
Rabbit polyclonal Collagen Type IV antibody
Product Categories/Family for anti-COL4A5 antibody
Applications Tested/Suitable for anti-COL4A5 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-COL4A5 antibody
IHC: 1:50-1:100
NCBI/Uniprot data below describe general gene information for COL4A5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
161,632 Da[Similar Products]
NCBI Official Full Name
collagen type IV alpha 5 chain, partial
NCBI Official Synonym Full Names
collagen, type IV, alpha 5
NCBI Official Symbol
COL4A5  [Similar Products]
NCBI Official Synonym Symbols
ATS; ASLN; CA54
  [Similar Products]
NCBI Protein Information
collagen alpha-5(IV) chain
UniProt Protein Name
Collagen alpha-5(IV) chain
Protein Family
UniProt Gene Name
COL4A5  [Similar Products]
UniProt Entry Name
CO4A5_HUMAN
NCBI Summary for COL4A5
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for COL4A5
COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Extracellular matrix

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction

Molecular Function: extracellular matrix structural constituent

Biological Process: axon guidance; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; neuromuscular junction development

Disease: Alport Syndrome, X-linked
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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