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anti-CYP17A1 antibody :: Goat CYP17A1 Polyclonal Antibody

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Catalog # MBS421228
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

CYP17A1, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-CYP17A1 Antibody

Product Synonym Names
CYP17A1; cytochrome P450, family 17, subfamily A, polypeptide 1; HGNC: 2593; CPT7; CYP17; P450C17; S17AH; cytochrome P450, family 17; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome p450 XVIIA1; steroid 17-a; CYP17A1 antibody; cytochrome P450; family 17; subfamily A; polypeptide 1 antibody; HGNC: 2593 antibody; CPT7 antibody; CYP17 antibody; P450C17 antibody; S17AH antibody; cytochrome P450; family 17 antibody; cytochrome P450; subfamily XVII (steroid 17-alpha-hydroxylase); adrenal hyperplasia antibody; cytochrome p450 XVIIA1 antibody; steroid 17-alpha-hydroxylase/17; 20 lyase antibody; steroid 17-alpha-monooxygenase antibody
Antibody/Peptide Pairs
CYP17A1 peptide (MBS425548) is used for blocking the activity of CYP17A1 antibody (MBS421228)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
508
Immunogen Sequence
LFKDGDQKLE K
OMIM
202110
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Cat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-LFKDGDQKLEK, from the internal region of the protein sequence according to NP_000093.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CYP17A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CYP17A1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CYP17A1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 60kDa band observed in human ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-CYP17A1 antibody
(0.3ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CYP17A1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CYP17A1 antibody
(3.8ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-CYP17A1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CYP17A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,371 Da
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450 family 17 subfamily A member 1
NCBI Official Symbol
CYP17A1  [Similar Products]
NCBI Official Synonym Symbols
CPT7; CYP17; S17AH; P450C17
  [Similar Products]
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Synonym Protein Names
17-alpha-hydroxyprogesterone aldolase (EC:4.1.2.30
UniProt Gene Name
CYP17A1  [Similar Products]
UniProt Synonym Gene Names
CYP17; S17AH; Cytochrome P450c17  [Similar Products]
UniProt Entry Name
CP17A_HUMAN
NCBI Summary for CYP17A1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP17A1
CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; EC 4.1.2.30; EC 1.14.99.9; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: axon; cell soma; endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrion

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; heme binding; iron ion binding; oxygen binding; steroid 17-alpha-monooxygenase activity

Biological Process: adrenal gland development; androgen biosynthetic process; biphenyl metabolic process; dibenzo-p-dioxin metabolic process; glucocorticoid biosynthetic process; hippocampus development; hormone biosynthetic process; Leydig cell differentiation; ovulation; phenol metabolic process; phthalate metabolic process; progesterone metabolic process; response to acetate; response to cAMP; response to cytokine stimulus; response to drug; response to herbicide; response to insecticide; response to ionizing radiation; response to methylmercury; response to nutrient levels; response to retinoic acid; response to steroid hormone stimulus; sex differentiation; steroid biosynthetic process; steroid metabolic process; sterol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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