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anti-KRT8 antibody :: Guinea pig Cytokeratin 8,18 Polyclonal Antibody

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Catalog # MBS621174
Unit / Price
  0.1 mL  /  $695 +1 FREE 8GB USB
anti-KRT8 antibody
Product Name

Cytokeratin 8,18 (KRT8), Polyclonal Antibody

Popular Item
Also Known As

Cytokeratin 8,18

Product Synonym Names
Anti -Cytokeratin 8,18
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (53290971..53343650, complement). Location: 12q13
OMIM
148060
3D Structure
ModBase 3D Structure for P05787
Clonality
Polyclonal
Isotype
IgG
Host
Guinea pig
Species Reactivity
Human, rat, mouse, Syrian hamster, Xenopus laevis, chicken.
Specificity
Recognizes bovine Cytokeratin 8,18 of simple type epithelium at ~52.5kD and 45.5kD respectively. Detected all adeno-Ca tested, undifferentiated CA, hepatocellular CA and cervix Ca tumors.
Purity/Purification
Serum
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Immunogen
Cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Other Notes
Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KRT8 antibody
Cytokeratins are a family of water-insoluble intermediate laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1-20) in both normal and malignant epithelial cell lines. Speci c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. Cytokeratins may also be clinically signi cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors.
Product Categories/Family for anti-KRT8 antibody
Applications Tested/Suitable for anti-KRT8 antibody
Suitable for use in ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Other applications have not been tested.
Application Notes for anti-KRT8 antibody
Western Blot: 1:2000
Immunohistochemistry (Frozen): 1:100.
Immunohistochemistry (Paraffin): 1:50. Microwave treatment recommended.

Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
cytokeratin 8 (279 AA)
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
  [Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Synonym Protein Names
Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CYK8; CK-8; K8  [Similar Products]
UniProt Entry Name
K2C8_HUMAN
NCBI Summary for KRT8
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for KRT8
K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; keratin filament; cytoplasm; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: viral reproduction; tumor necrosis factor-mediated signaling pathway; response to other organism; sarcomere organization; response to hydrostatic pressure

Disease: Cirrhosis, Familial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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