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anti-PRKAA1 antibody :: Rabbit AMPK alpha 1/2 Polyclonal Antibody

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Catalog # MBS8240942
Unit / Price
  0.03 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $245 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

AMPK alpha 1/2 (PRKAA1), Polyclonal Antibody

Popular Item
Also Known As

Anti-AMPK alpha 1/2 (pT183/172) Antibody

Product Synonym Names
PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
574
OMIM
602739
3D Structure
ModBase 3D Structure for Q13131
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chicken, Porcine
Specificity
Recognizes endogenous levels of AMPK alpha 1/2 (pT183/172) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AMPK alpha 1/2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-PRKAA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PRKAA1 antibody
Rabbit polyclonal antibody to AMPK alpha 1/2 (pT183/172)
Applications Tested/Suitable for anti-PRKAA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-PRKAA1 antibody
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-PRKAA1 antibody
Western blot analysis of AMPK alpha 1/2 (pT183/172) expression in Jurkat Adriamycin-treated (A), K562 Adriamycin-treated (B) whole cell lysates.
anti-PRKAA1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PRKAA1 antibody
Immunohistochemical analysis of AMPK alpha 1/2 (pT183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-PRKAA1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PRKAA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,523 Da
NCBI Official Full Name
5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1  [Similar Products]
NCBI Official Synonym Symbols
AMPK; AMPKa1
  [Similar Products]
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Synonym Protein Names
Acetyl-CoA carboxylase kinase (EC:2.7.11.27); ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31); HMGCR kinase; Tau-protein kinase PRKAA1 (EC:2.7.11.26)
UniProt Gene Name
PRKAA1  [Similar Products]
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase  [Similar Products]
UniProt Entry Name
AAPK1_HUMAN
NCBI Summary for PRKAA1
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Comments for PRKAA1
AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; Autophagy; EC 2.7.11.26; EC 2.7.11.1; EC 2.7.11.31; EC 2.7.11.27; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: nucleoplasm; cytoplasm; apical plasma membrane; intracellular; cytosol; nucleus; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein C-terminus binding; cAMP-dependent protein kinase activity; metal ion binding; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; protein kinase activity; protein binding; tau-protein kinase activity; chromatin binding; kinase binding; ATP binding; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

Biological Process: activation of MAPK activity; protein heterooligomerization; lipid biosynthetic process; rhythmic process; positive regulation of cholesterol biosynthetic process; glucose homeostasis; signal transduction; protein amino acid phosphorylation; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to gamma radiation; cell cycle arrest; negative regulation of glucosylceramide biosynthetic process; positive regulation of autophagy; fatty acid biosynthetic process; response to UV; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; regulation of circadian rhythm; cholesterol biosynthetic process; fatty acid homeostasis; cold acclimation; positive regulation of glycolysis; insulin receptor signaling pathway; response to hypoxia; autophagy; fatty acid oxidation; response to activity; negative regulation of apoptosis
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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