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Rabbit Phosphotyrosine Polyclonal Antibody

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Catalog # MBS472051
Unit / Price
  0.1 mL  /  $170 +1 FREE 8GB USB
Product Name

Phosphotyrosine, Polyclonal Antibody

Popular Item
Also Known As

Anti-Phosphotyrosine

Product Synonym Names
Phosphotyrosine; Ptyr
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken
Specificity
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Immunogen
Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-Phosphotyrosine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Phosphotyrosine antibody
Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications.Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
Applications Tested/Suitable for anti-Phosphotyrosine antibody
ELISA, Western Blot, ICC, IP
Application Notes for anti-Phosphotyrosine antibody
ELISA: 1:2000
Immunoprecipitation: 1:100
Western Blot: 1:1000
ICC: 1:100

Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine and mouse monoclonal anti-Phosphotyrosine , then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
anti-Phosphotyrosine antibody Testing Data image
Bar graph showing rabbit polyclonal anti-Phosphotyrosine binding to a variety of phosphotyrosine containing peptides, but no binding to unphosphorylated peptide (beta-Catenin (a.a. 29-45).
anti-Phosphotyrosine antibody Testing Data image
Product References and Citations for anti-Phosphotyrosine antibody
• Ross, A.H. et al. (1981) Nature 294:654.
• Wang, J.Y.J. (1988) Anal. Biochem 172:1.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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