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anti-ACO1 antibody :: Rabbit ACO1 Polyclonal Antibody

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Catalog # MBS3205193
Unit / Price
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  0.1 mL  /  $365 +1 FREE 8GB USB
Product Name

ACO1, Polyclonal Antibody

Full Product Name

ACO1 antibody - N-terminal region

Product Synonym Gene Name
IRP1; ACONS; IREB1; IREBP; IREBP1[Similar Products]
Antibody/Peptide Pairs
ACO1 peptide (MBS3230158) is used for blocking the activity of ACO1 antibody (MBS3205193)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: MSNPFAHLAE PLDPVQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAIRNC
3D Structure
ModBase 3D Structure for P28271
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Cow: 100%; Dog: 93%; Guinea Pig: 91%; Horse: 92%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 86%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human ACO1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ACO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACO1 antibody
This is a rabbit polyclonal antibody against ACO1. It was validated on Western Blot and immunohistochemistry

Target Description: ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
Applications Tested/Suitable for anti-ACO1 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-ACO1 antibody
Rabbit Anti-ACO1 Antibody
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
anti-ACO1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ACO1 antibody
Sample Type :
Human Capan1 cells (Pancreatic cancer cell line)
Primary Antibody Dilution :
1:300
Secondary Antibody :
Anti-rabbit-AlexaFluor-488
Secondary Antibody Dilution :
1:200
Color/Signal Descriptions :
ACO1: Green DAPI: Blue
Gene Name :
ACO1
Submitted by :
Dr. Pankaj Singh, UNMC, Omaha, NE
anti-ACO1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-ACO1 antibody
Sample Type: HePG2
Lanes :
Lane 1: 100ug HePG2 lysate
Primary Antibody Dilution :
1:1000
Secondary Antibody :
Goat anti rabbit-HRP
Secondary Antibody Dilution :
1:8000
Gene Name :
ACO1
Submitted by :
José Antonio Bárcena, University of Córdoba
anti-ACO1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACO1 antibody
Host: Rabbit
Target Name: ACO1
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml
anti-ACO1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACO1 antibody
Host: Rabbit
Target Name: ACO1
Sample Type: Fetal Kidney
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/Lane
Gel Concentration: 0.12
anti-ACO1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACO1 antibody
Lanes:
1. 45ug capan1 cell lysate
2. 45 ug HPAF cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
ACO1
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE

anti-ACO1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACO1 antibody
Lanes:
1. 60 ug mouse liver extract 2. 60 ug mouse liver extract 3. 60 ug mouse liver extract
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:3000
Gene Name:
ACO1
Submitted by:
Dr. Hao Zhu, University of Kansas Medical Center

anti-ACO1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACO1 antibody
WB Suggested Anti-ACO1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human kidney
anti-ACO1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ACO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
98kDa
NCBI Official Full Name
cytoplasmic aconitate hydratase
NCBI Official Synonym Full Names
aconitase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
  [Similar Products]
NCBI Protein Information
cytoplasmic aconitate hydratase
UniProt Protein Name
Cytoplasmic aconitate hydratase
UniProt Synonym Protein Names
Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Ireb1; Irebp; Aconitase; IRP1; IRE-BP 1  [Similar Products]
UniProt Entry Name
ACOC_MOUSE
NCBI Summary for ACO1
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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