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anti-RAB8A antibody :: Goat RAB8A Polyclonal Antibody

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Catalog # MBS421586
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

RAB8A, Polyclonal Antibody

Full Product Name

Goat anti-RAB8A Antibody

Product Synonym Names
RAB8A; RAB8A, member RAS oncogene family; MEL; RAB8; mel transforming oncogene; mel transforming oncogene (RAB8 homolog); mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; ras-a; RAB8A antibody; RAB8A; member RAS oncogene family antibody; MEL antibody; RAB8 antibody; mel transforming oncogene antibody; mel transforming oncogene (RAB8 homolog) antibody; mel transforming oncogene (derived from cell line NK14) antibody; mel transforming oncogene (derived from cell line NK14)- RAB8 homolog antibody; ras-associated protein RAB8 antibody
Antibody/Peptide Pairs
RAB8A peptide (MBS426539) is used for blocking the activity of RAB8A antibody (MBS421586)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
100ug specific antibody in 200ul (lot specific)
Peptide with sequence C-NQGVKITPDQQKR, from the C Terminus of the protein sequence according to NP_005361.2.
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-RAB8A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-RAB8A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-RAB8A antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 25kDa band observed in Human Brain (Cerebellum), Kidney and Heart lysates (calculated MW of 23.7kDa according to NP_005361.2). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-RAB8A antibody
(0.1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-RAB8A antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for RAB8A. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,564 Da
NCBI Official Full Name
ras-related protein Rab-8A
NCBI Official Synonym Full Names
RAB8A, member RAS oncogene family
NCBI Official Symbol
RAB8A  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
ras-related protein Rab-8A
UniProt Protein Name
Ras-related protein Rab-8A
UniProt Synonym Protein Names
Oncogene c-mel
Protein Family
UniProt Gene Name
RAB8A  [Similar Products]
UniProt Synonym Gene Names
MEL; RAB8  [Similar Products]
UniProt Entry Name
NCBI Summary for RAB8A
The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]
UniProt Comments for RAB8A
RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: Oncoprotein; G protein, monomeric, Rab; G protein, monomeric; G protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cell soma; centriole; centrosome; cilium; cytoplasmic vesicle membrane; cytosol; dendrite; Golgi membrane; nonmotile primary cilium; nucleolus; nucleus; phagocytic vesicle; phagocytic vesicle membrane; plasma membrane; postsynaptic density; recycling endosome membrane; synaptic vesicle; trans-Golgi network transport vesicle

Molecular Function: GDP binding; GTP binding; GTPase activity; myosin V binding; protein binding; protein kinase binding; Rab GTPase binding

Biological Process: axonogenesis; cellular response to insulin stimulus; cilium biogenesis; G2/M transition of mitotic cell cycle; Golgi vesicle fusion to target membrane; intracellular protein transport; metabolic process; nucleocytoplasmic transport; protein secretion; regulation of long-term neuronal synaptic plasticity; regulation of protein transport; small GTPase mediated signal transduction; vesicle docking during exocytosis
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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