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anti-TFE3 antibody :: Goat TFE3 Polyclonal Antibody

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Catalog # MBS420221
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TFE3, Polyclonal Antibody

Also Known As

Goat anti-TFE3 Antibody

Product Synonym Names
TFE3; transcription factor binding to IGHM enhancer 3; Transcription factor for IgH enhancer; RCCP2; TFEA; bHLHe33; OTTHUMP00000024272; Renal cell carcinoma, papillary; Transcription factor for IgH enhancer; transcription factor E family, member A; transc; TFE3 antibody; transcription factor binding to IGHM enhancer 3 antibody; Transcription factor for IgH enhancer antibody; RCCP2 antibody; TFEA antibody; bHLHe33 antibody; OTTHUMP00000024272 antibody; Renal cell carcinoma; papillary antibody; Transcription factor for IgH enhancer antibody; transcription factor E family; member A antibody; transcription factor E3 antibody
Antibody/Peptide Pairs
TFE3 peptide (MBS426553) is used for blocking the activity of TFE3 antibody (MBS420221)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
575
Immunogen Sequence
SHAAEPARDG VEAS
OMIM
314310
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence SHAAEPARDGVEAS-C, from the N Terminus of the protein sequence according to NP_006512.2.
Epitope
N Terminus
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5958
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-TFE3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TFE3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-TFE3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 60kDa band observed in lysates of HEK293 (calculated MW of 61.5kDa according to NP_006512.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-TFE3 antibody
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-TFE3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TFE3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,363 Da
NCBI Official Full Name
transcription factor E3 isoform 1
NCBI Official Synonym Full Names
transcription factor binding to IGHM enhancer 3
NCBI Official Symbol
NCBI Official Synonym Symbols
TFEA; RCCP2; RCCX1; bHLHe33
  [Similar Products]
NCBI Protein Information
transcription factor E3
UniProt Protein Name
Transcription factor E3
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 33; bHLHe33
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BHLHE33; bHLHe33  [Similar Products]
UniProt Entry Name
TFE3_HUMAN
NCBI Summary for TFE3
This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Comments for TFE3
TFE3: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. Belongs to the MiT/TFE family.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: adaptive immune response; humoral immune response; positive regulation of cell adhesion; positive regulation of transcription from RNA polymerase II promoter; regulation of osteoclast differentiation; transcription from RNA polymerase II promoter

Disease: Renal Cell Carcinoma, Xp11-associated
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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