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anti-ADH5 antibody :: Goat ADH5 Polyclonal Antibody

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Catalog # MBS420931
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ADH5, Polyclonal Antibody

Also Known As

Goat anti-ADH5 Antibody

Product Synonym Names
ADH5; alcohol dehydrogenase 5 (class III), chi polypeptide; ADH-3; ADHX; FDH; Alcohol dehydrogenase (class III), chi polypeptide; class III alcohol dehydrogenase 5 chi subunit; formaldehyde dehydrogenase; glutathione-dependent formaldehyde dehydrogenase; ADH5 antibody; alcohol dehydrogenase 5 (class III); chi polypeptide antibody; ADH-3 antibody; ADHX antibody; FDH antibody; Alcohol dehydrogenase (class III); chi polypeptide antibody; class III alcohol dehydrogenase 5 chi subunit antibody; formaldehyde dehydrogenase antibody; glutathione-dependent formaldehyde dehydrogenase antibody; GSNOR antibody
Antibody/Peptide Pairs
ADH5 peptide (MBS425283) is used for blocking the activity of ADH5 antibody (MBS420931)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
374
Immunogen Sequence
KKIKVDEFVT HN
OMIM
103710
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KKIKVDEFVTHN, from the internal region of the protein sequence according to NP_000662.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ADH5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ADH5 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ADH5 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: An anonymous customer found positive results in IHC on Rat penile tissue.
Western Blot: Approx 38kDa band observed in Human Testis lysates (calculated MW of 39.7kDa according to NP_000662.3). Recommended concentration: 0.5-1.5ug/ml. An anonymous customer found positive results in WB on Rat penis tissue. An anonymous customer found positive results in WB on Mouse penile tissue.

Western Blot (WB) of anti-ADH5 antibody
(0.5ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ADH5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ADH5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,724 Da
NCBI Official Full Name
alcohol dehydrogenase class-3
NCBI Official Synonym Full Names
alcohol dehydrogenase 5 (class III), chi polypeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p
  [Similar Products]
NCBI Protein Information
alcohol dehydrogenase class-3
UniProt Protein Name
Alcohol dehydrogenase class-3
UniProt Synonym Protein Names
Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-); FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FALDH; FDH; GSH-FDH  [Similar Products]
UniProt Entry Name
ADHX_HUMAN
NCBI Summary for ADH5
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
UniProt Comments for ADH5
ADH5: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Protein type: Lipid Metabolism - fatty acid; Amino Acid Metabolism - tyrosine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.1.1.284; Oxidoreductase; Energy Metabolism - methane; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.1; Mitochondrial; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol; mitochondrion

Molecular Function: alcohol dehydrogenase activity; electron carrier activity; fatty acid binding; formaldehyde dehydrogenase activity; protein homodimerization activity; S-(hydroxymethyl)glutathione dehydrogenase activity; zinc ion binding

Biological Process: ethanol oxidation; formaldehyde catabolic process; peptidyl-cysteine S-nitrosylation; positive regulation of blood pressure; respiratory system process; response to lipopolysaccharide; response to nitrosative stress; response to redox state; retinoid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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