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anti-ADRA1B antibody :: Goat ADRA1B Polyclonal Antibody

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Catalog # MBS423030
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

ADRA1B, Polyclonal Antibody

Also Known As

Goat anti-ADRA1B Antibody

Product Synonym Names
Goat Anti-ADRA1B (aa252-264) Antibody; ADRA1B; adrenergic, alpha-1B-, receptor; ADRA1; ALPHA1BAR; OTTHUMP00000160844; alpha-1B adrenergic receptor; alpha-1B adrenoceptor; alpha-1B adrenoreceptor; alpha-1B-adrenergic receptor; ADRA1B antibody; adrenergic; alpha-1B-; receptor antibody; ADRA1 antibody; ALPHA1BAR antibody; OTTHUMP00000160844 antibody; alpha-1B adrenergic receptor antibody; alpha-1B adrenoceptor antibody; alpha-1B adrenoreceptor antibody; alpha-1B-adrenergic receptor antibody
Antibody/Peptide Pairs
ADRA1B peptide (MBS428280) is used for blocking the activity of ADRA1B antibody (MBS423030)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
520
Immunogen Sequence
SSTKAKGHNP RSS
OMIM
104220
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SSTKAKGHNPRSS, from the internal region of the protein sequence according to NP_000670.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ADRA1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ADRA1B antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ADRA1B antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 56.8kDa according to NP_000670.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-ADRA1B antibody
(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ADRA1B antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ADRA1B antibody
(0.1ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ADRA1B antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ADRA1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,836 Da
NCBI Official Full Name
alpha-1B adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor alpha 1B
NCBI Official Symbol
ADRA1B  [Similar Products]
NCBI Official Synonym Symbols
ADRA1; ALPHA1BAR
  [Similar Products]
NCBI Protein Information
alpha-1B adrenergic receptor
UniProt Protein Name
Alpha-1B adrenergic receptor
UniProt Synonym Protein Names
Alpha-1B adrenoreceptor; Alpha-1B adrenoceptor
UniProt Gene Name
ADRA1B  [Similar Products]
UniProt Synonym Gene Names
Alpha-1B adrenoceptor  [Similar Products]
UniProt Entry Name
ADA1B_HUMAN
NCBI Summary for ADRA1B
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [provided by RefSeq, Jul 2008]
UniProt Comments for ADRA1B
ADRA1B: a G protein-coupled catecholamine receptor that activates mitogenic responses and regulates growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. Can induce neoplastic transformation when transfected into cell lines. Can regulate the phosphorylation status of the STAT3 pathway. Mediates blood pressure and aorta contractile responses induced by alpha-1 agonists. Activates a phosphatidylinositol second messenger system.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to plasma membrane; nuclear membrane; nucleus; plasma membrane

Molecular Function: alpha1-adrenergic receptor activity; protein heterodimerization activity

Biological Process: cell proliferation; cell-cell signaling; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); glucose homeostasis; multicellular organismal development; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; positive regulation of MAPKKK cascade; positive regulation of smooth muscle contraction; positive regulation of vasoconstriction
Research Articles on ADRA1B
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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