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anti-ALDH18A1 antibody :: Goat ALDH18A1 Polyclonal Antibody

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Catalog # MBS422476
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

ALDH18A1, Polyclonal Antibody

Also Known As

Goat anti-ALDH18A1 Antibody

Product Synonym Names
ALDH18A1; aldehyde dehydrogenase 18 family, member A1; GSAS; MGC117316; P5CS; PYCS; aldehyde dehydrogenase 18A1; delta1-pyrroline-5-carboxlate synthetase; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase); aldehyde dehydrogenase 18 family; member A1 antibody; aldehyde dehydrogenase 18A1 antibody; delta1-pyrroline-5-carboxlate synthetase antibody; GSAS antibody; MGC117316 antibody; P5CS antibody; PYCS antibody; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase) antibody; ALDH18A1 antibody
Antibody/Peptide Pairs
ALDH18A1 peptide (MBS427422) is used for blocking the activity of ALDH18A1 antibody (MBS422476)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
795
Immunogen Sequence
SEHGSLKYLH
OMIM
138250
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SEHGSLKYLH, from the C Terminus of the protein sequence according to NP_002851.2; NP_001017423.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_002851.2; NP_001017423.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ALDH18A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ALDH18A1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ALDH18A1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 85kDa band observed in lysates of cell lines NIH3T3 and MCF7 (calculated MW of 87.3kDa according to NP_002851.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-ALDH18A1 antibody
(1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ALDH18A1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ALDH18A1 antibody
(0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ALDH18A1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ALDH18A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001017423.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
87,089 Da
NCBI Official Full Name
delta-1-pyrroline-5-carboxylate synthase isoform 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 18 family member A1
NCBI Official Symbol
ALDH18A1  [Similar Products]
NCBI Official Synonym Symbols
GSAS; P5CS; PYCS; ADCL3; SPG9A; SPG9B; ARCL3A
  [Similar Products]
NCBI Protein Information
delta-1-pyrroline-5-carboxylate synthase
UniProt Protein Name
Delta-1-pyrroline-5-carboxylate synthase
UniProt Synonym Protein Names
Aldehyde dehydrogenase family 18 member A1Including the following 2 domains:Glutamate 5-kinase (EC:2.7.2.11); GKAlternative name(s):Gamma-glutamyl kinase
UniProt Gene Name
ALDH18A1  [Similar Products]
UniProt Synonym Gene Names
GSAS; P5CS; PYCS; P5CS; GK; GPR  [Similar Products]
UniProt Entry Name
P5CS_HUMAN
NCBI Summary for ALDH18A1
This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ALDH18A1
ALDH18A1: Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A). A syndrome characterized by facial dysmorphism with a progeroid appearance, large and late-closing fontanel, cutis laxa, joint hyperlaxity, athetoid movements and hyperreflexia, pre- and postnatal growth retardation, intellectual deficit, developmental delay, and ophthalmologic abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Kinase, other; EC 2.7.2.11; Mitochondrial; EC 1.2.1.41; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion

Molecular Function: ATP binding; delta1-pyrroline-5-carboxylate synthetase activity; glutamate 5-kinase activity; glutamate-5-semialdehyde dehydrogenase activity; protein binding

Biological Process: amino acid biosynthetic process; citrulline biosynthetic process; glutamate metabolic process; ornithine biosynthetic process; phosphorylation; proline biosynthetic process

Disease: Cutis Laxa, Autosomal Dominant 3; Cutis Laxa, Autosomal Recessive, Type Iiia; Spastic Paraplegia 9a, Autosomal Dominant; Spastic Paraplegia 9b, Autosomal Recessive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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