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anti-SETDB2 antibody :: Goat CLLD8/SETDB2 Polyclonal Antibody

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Catalog # MBS421496
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

CLLD8/SETDB2, Polyclonal Antibody

Full Product Name

Goat anti-CLLD8/SETDB2 Antibody

Product Synonym Names
Goat Anti-SETDB2 Antibody; SETDB2; CLLD8; SET domain, bifurcated 2; CLLL8 protein; C13orf4; CLLL8; DKFZp586I0123; DKFZp761J1217; KMT1F; chronic lymphocytic leukemia deletion region 8; lysine N-methyltransferase 1F; histone-lysine N-methyltransferase SETDB2; SETDB2 antibody; CLLD8 antibody; SET domain; bifurcated 2 antibody; CLLL8 protein antibody; C13orf4 antibody; CLLL8 antibody; DKFZp586I0123 antibody; DKFZp761J1217 antibody; KMT1F antibody; chronic lymphocytic leukemia deletion region 8 antibody; lysine N-methyltransferase 1F antibody; histone-lysine N-methyltransferase SETDB2 antibody; CLLD8 / SETDB2
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
GEKNGDAKTF WME
OMIM
607865
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence GEKNGDAKTFWME-C, from the N Terminus of the protein sequence according to NP_114121.2; NP_001153780.1.
Epitope
N Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SETDB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SETDB2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SETDB2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 80kDa band observed in Human Heart lysates (calculated MW of 81.9kDa according to NP_114121.1). Recommended concentration: 1.5-4.5ug/ml. This product is not suitable in WB on Mouse Heart and Rat Heart lysates.

Western Blot (WB) of anti-SETDB2 antibody
(1.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SETDB2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SETDB2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001153780.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,766 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SETDB2 isoform a
NCBI Official Synonym Full Names
SET domain bifurcated 2
NCBI Official Symbol
SETDB2  [Similar Products]
NCBI Official Synonym Symbols
CLLD8; CLLL8; KMT1F; C13orf4
  [Similar Products]
NCBI Protein Information
histone-lysine N-methyltransferase SETDB2
UniProt Protein Name
Histone-lysine N-methyltransferase SETDB2
UniProt Synonym Protein Names
Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2
UniProt Gene Name
SETDB2  [Similar Products]
UniProt Synonym Gene Names
C13orf4; CLLD8; KMT1F  [Similar Products]
UniProt Entry Name
SETB2_HUMAN
NCBI Summary for SETDB2
This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Comments for SETDB2
SETDB2: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: chromosome; nucleoplasm; nucleus

Molecular Function: DNA binding; histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; protein binding; zinc ion binding

Biological Process: cell division; chromosome segregation; heart looping; histone H3-K9 methylation; mitosis; negative regulation of transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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