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anti-CRTC2 antibody :: Goat CRTC2 Polyclonal Antibody

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Catalog # MBS422613
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

CRTC2, Polyclonal Antibody

Also Known As

Goat anti-CRTC2 Antibody

Product Synonym Names
Goat Anti-CRTC2 / TORC2 (aa 83-96) Antibody; Crtc2; CREB regulated transcription coactivator 2; 4632407F12Rik; Torc2; OTTMUSP00000023505; transducer of regulated cAMP response element-binding protein (CREB) 2; Crtc2 antibody; CREB regulated transcription coactivator 2 antibody; 4632407F12Rik antibody; Torc2 antibody; OTTMUSP00000023505 antibody; transducer of regulated cAMP response element-binding protein (CREB) 2 antibody; CRTC2
Antibody/Peptide Pairs
CRTC2 peptide (MBS428267) is used for blocking the activity of CRTC2 antibody (MBS422613)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
692
Immunogen Sequence
DPAVEDSFRS DRLQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DPAVEDSFRSDRLQ, from the C Terminus of the protein sequence according to NP_083157.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CRTC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CRTC2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CRTC2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 73.2kDa according to NP_083157.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-CRTC2 antibody
(1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CRTC2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CRTC2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,216 Da
NCBI Official Full Name
CREB-regulated transcription coactivator 2
NCBI Official Synonym Full Names
CREB regulated transcription coactivator 2
NCBI Official Symbol
Crtc2  [Similar Products]
NCBI Official Synonym Symbols
Torc2; mTORC2; 4632407F12Rik
  [Similar Products]
NCBI Protein Information
CREB-regulated transcription coactivator 2
UniProt Protein Name
CREB-regulated transcription coactivator 2
UniProt Synonym Protein Names
Transducer of regulated cAMP response element-binding protein 2; TORC-2; Transducer of CREB protein 2
UniProt Gene Name
Crtc2  [Similar Products]
UniProt Synonym Gene Names
Torc2; TORC-2; Transducer of CREB protein 2  [Similar Products]
UniProt Entry Name
CRTC2_MOUSE
UniProt Comments for CRTC2
TORC2: the transducer of regulated CREB protein 2 (TORC2) is a cAMP responsive coactivator that, in concert with LKB1 and AMPK, controls glucose homeostasis in the liver. Under fasting conditions, cytoplasmic TORC2 is transported to the nucleus where it binds to CREB and stimulates gluconeogenisis. Re-feeding and the ensuing insulin response inhibits gluconeogenic gene expression by promoting the phosphorylation, cytoplasmic export and degradation of TORC2. TORC2 is phosphorylated by the kinase QIK which in turn is activated by phosphorylation at Ser358 by insulin-activated Akt2. Phosphorylated TORC2 is subsequently ubiquitinylated at Lys 628 and degraded by the 26S proteasome. TORC2 protein levels and activity are increased in diabetes owing to a block in TORC2 phosphorylation, implicating this pathway in the pathogenesis of diabetes. The CREB/TORC2 regulatory axis controls the normal pattern of germinal center (GC) B cell gene activation/repression that promotes B cell development and circumvents GC lymphomagenesis.

Protein type: Transcription, coactivator/corepressor

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: cAMP response element binding protein binding; chromatin binding; protein binding

Biological Process: activation of CREB transcription factor; gluconeogenesis; glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; protein homotetramerization; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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