• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-ERBB3 antibody :: Goat ERBB3/HER3 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS420204
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ERBB3/HER3, Polyclonal Antibody

Also Known As

Goat anti-ERBB3/HER3 Antibody

Product Synonym Names
ERBB3; HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); HGNC: 3431; ErbB-3; MDA-BF-1; c-erbB-3; c-erbB3; erbB3-S; p180-ErbB3; p45-sErbB3; p85-sErbB3; erbB-3; LCCS2; MGC88033; receptor tyrosine-protein kinase erbB-3; v-erb-b2 avian; ERBB3 antibody; HER3 antibody; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody; HGNC: 3431 antibody; ErbB-3 antibody; MDA-BF-1 antibody; c-erbB-3 antibody; c-erbB3 antibody; erbB3-S antibody; p180-ErbB3 antibody; p45-sErbB3 antibody; p85-sErbB3 antibody; erbB-3 antibody; LCCS2 antibody; MGC88033 antibody; receptor tyrosine-protein kinase erbB-3 antibody; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody; ERBB3 / HER3
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1342
Immunogen Sequence
HSRLFPKANA QRT
OMIM
190151
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-HSRLFPKANAQRT, from the C Terminus of the protein sequence according to NP_001973.2.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 1 (NP_001973.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ERBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ERBB3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ERBB3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 150kDa band observed in human lysates of T cell line JURKAT (calculated MW of 148kDa according to NP_001973.2). Recommended concentration: 0.1-0.3ug/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-ERBB3 antibody
(0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ERBB3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ERBB3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
77,426 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-3 isoform 1
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 3
NCBI Official Symbol
ERBB3  [Similar Products]
NCBI Official Synonym Symbols
HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
  [Similar Products]
NCBI Protein Information
receptor tyrosine-protein kinase erbB-3
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-3
UniProt Synonym Protein Names
Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3
UniProt Gene Name
ERBB3  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ERBB3_HUMAN
NCBI Summary for ERBB3
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for ERBB3
HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: apical plasma membrane; basolateral plasma membrane; extracellular space; integral to plasma membrane; intracellular; lateral plasma membrane; plasma membrane; receptor complex

Molecular Function: ATP binding; growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein tyrosine kinase activator activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor activity

Biological Process: cranial nerve development; heart development; MAPKKK cascade; negative regulation of cell adhesion; negative regulation of neuron apoptosis; negative regulation of secretion; negative regulation of signal transduction; neuron apoptosis; peptidyl-tyrosine phosphorylation; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; Schwann cell differentiation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing

Disease: Lethal Congenital Contracture Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000