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anti-GAD1 antibody :: Goat GAD25/GAD67 Polyclonal Antibody

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Catalog # MBS422705
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

GAD25/GAD67 (GAD1), Polyclonal Antibody

Also Known As

Goat anti-GAD25/GAD67 Antibody

Product Synonym Names
Goat Anti-GAD1 (isoform GAD67) Antibody; GAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP; 67 kDa glutamic acid decarboxylase; GAD-67; OTTHUMP00000204990; OTTHUMP00000204993; OTTHUMP00000204994; OTTHUMP00000204995; glutamate decarboxylase 1; glutamate decarboxylase 67 kDa isofo; 67 kDa glutamic acid decarboxylase antibody; FLJ45882 antibody; GAD antibody; GAD-67 antibody; glutamate decarboxylase 1 antibody; glutamate decarboxylase 1 (brain; 67kDa) antibody; glutamate decarboxylase 67 kDa isoform antibody; OTTHUMP00000204990 antibody; OTTHUMP00000204993 antibody; OTTHUMP00000204994 antibody; OTTHUMP00000204995 antibody; SCP antibody; GAD1 antibody; GAD25 / GAD67
Product Synonym Gene Name
GAD25; GAD67[Similar Products]
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
224
Immunogen Sequence
QRTNSLEEKS R
OMIM
603513
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QRTNSLEEKSR, from the internal region of the protein sequence according to NP_038473.2; NP_000808.2.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_038473.2; NP_000808.2).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GAD1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GAD1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 70kDa band observed in Mouse Brain lysates (calculated MW of 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-GAD1 antibody
(1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-GAD1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_000808.2[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,440 Da
NCBI Official Full Name
glutamate decarboxylase 1 isoform GAD25
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
  [Similar Products]
NCBI Protein Information
glutamate decarboxylase 1
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GAD; GAD67; GAD-67  [Similar Products]
UniProt Entry Name
DCE1_HUMAN
NCBI Summary for GAD1
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
UniProt Comments for GAD1
GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: intracellular; plasma membrane; presynaptic active zone; vesicle membrane

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein binding; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: gamma-aminobutyric acid biosynthetic process; glutamate catabolic process; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process; neurotransmitter secretion; protein-pyridoxal-5-phosphate linkage; response to drug; synaptic transmission

Disease: Cerebral Palsy, Spastic Quadriplegic, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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