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anti-HMGA1 antibody :: Goat HMGA1 Polyclonal Antibody

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Catalog # MBS422168
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

HMGA1, Polyclonal Antibody

Full Product Name

Goat anti-HMGA1 (aa9-21) Antibody

Product Synonym Names
Goat Anti-HMGA1 Antibody; HMGA1; high mobility group AT-hook 1; HMG-R; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; OTTHUMP00000016222; OTTHUMP00000016223; OTTHUMP00000016224; OTTHUMP00000039618; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; nonhistone chrom; HMGA1 antibody; high mobility group AT-hook 1 antibody; HMG-R antibody; HMGA1A antibody; HMGIY antibody; MGC12816 antibody; MGC4242 antibody; MGC4854 antibody; OTTHUMP00000016222 antibody; OTTHUMP00000016223 antibody; OTTHUMP00000016224 antibody; OTTHUMP00000039618 antibody; high-mobility group (nonhistone chromosomal) protein isoforms I and Y antibody; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y antibody; HMGA1 (aa9-21)
Antibody/Peptide Pairs
HMGA1 peptide (MBS425466) is used for blocking the activity of HMGA1 antibody (MBS422168)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
SQPLASKQEK DGT
OMIM
600701
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SQPLASKQEKDGT, from the internal region (near N Terminus) of the protein sequence according to NP_665906.1; NP_002122.1.
Epitope
Internal region (near N Terminus)
Note
This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-HMGA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HMGA1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HMGA1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 24kDa band observed in Human Pancreas (calculated MW of 11.7kDa/10.7kDa according to NP_665906.1; NP_002122.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1ug/ml.

Western Blot (WB) of anti-HMGA1 antibody
(0.5ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
anti-HMGA1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HMGA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_002122.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,694 Da
NCBI Official Full Name
high mobility group protein HMG-I/HMG-Y isoform a
NCBI Official Synonym Full Names
high mobility group AT-hook 1
NCBI Official Symbol
HMGA1  [Similar Products]
NCBI Official Synonym Symbols
HMG-R; HMGIY; HMGA1A
  [Similar Products]
NCBI Protein Information
high mobility group protein HMG-I/HMG-Y
UniProt Protein Name
High mobility group protein HMG-I/HMG-Y
UniProt Synonym Protein Names
High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein R
UniProt Gene Name
HMGA1  [Similar Products]
UniProt Synonym Gene Names
HMGIY; HMG-I(Y); High mobility group protein A1  [Similar Products]
UniProt Entry Name
HMGA1_HUMAN
NCBI Summary for HMGA1
This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]
UniProt Comments for HMGA1
HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytosol; focal adhesion; nucleoplasm; nucleus; transcription factor complex

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; AT DNA binding; chromatin binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; peroxisome proliferator activated receptor binding; protein binding; retinoic acid receptor binding; retinoid X receptor binding; transcription factor activity; transcription factor binding

Biological Process: base-excision repair; DNA unwinding during replication; negative regulation of cell proliferation; negative regulation of chromatin silencing; negative regulation of transcription, DNA-dependent; nuclear transport; nucleosome disassembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of transcription, DNA-dependent; response to virus; transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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