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anti-NEFM antibody :: Goat NEFM/NF-M Polyclonal Antibody

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Catalog # MBS421706
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

NEFM/NF-M, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-NEFM/NF-M Antibody

Product Synonym Names
NEFM; neurofilament, medium polypeptide; NEF3; NF-M; NFM; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NEFM antibody; neurofilament; medium polypeptide antibody; NEF3 antibody; NF-M antibody; NFM antibody; neurofilament triplet M protein antibody; neurofilament; medium polypeptide 150kDa antibody; neurofilament-3 (150 kD medium) antibody; NEFM / NF-M
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
916
Immunogen Sequence
QASHITVERK DYLK
OMIM
162250
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2; NP_001099011.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_005373.2; NP_001099011.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-NEFM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NEFM antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NEFM antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-NEFM antibody
(0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-NEFM antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NEFM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001099011.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,468 Da
NCBI Official Full Name
neurofilament medium polypeptide isoform 1
NCBI Official Synonym Full Names
neurofilament, medium polypeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
NFM; NEF3; NF-M
  [Similar Products]
NCBI Protein Information
neurofilament medium polypeptide
UniProt Protein Name
Neurofilament medium polypeptide
UniProt Synonym Protein Names
160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
UniProt Gene Name
UniProt Synonym Gene Names
NEF3; NFM; NF-M  [Similar Products]
UniProt Entry Name
NFM_HUMAN
NCBI Summary for NEFM
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
UniProt Comments for NEFM
NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: axon; neurofilament

Molecular Function: microtubule binding; protein binding; structural constituent of cytoskeleton

Biological Process: neurofilament bundle assembly
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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