• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-POU2F1 antibody :: Goat POU2F1/OCT1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS422485
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

POU2F1/OCT1, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-POU2F1/OCT1 Antibody

Product Synonym Names
POU2F1; POU class 2 homeobox 1; OCT1; OTF1; OTTHUMP00000032348; Octamer-binding transcription factor-1; POU domain, class 2, transcription factor 1; OCT1 antibody; Octamer-binding transcription factor-1 antibody; OTF1 antibody; OTTHUMP00000032348 antibody; POU class 2 homeobox 1 antibody; POU domain; class 2; transcription factor 1 antibody; POU2F1 antibody; POU2F1 / OCT1
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
743
Immunogen Sequence
NVQSKSNEES GDSQQ
OMIM
164175
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-NVQSKSNEESGDSQQ, from the internal region (near N Terminus) of the protein sequence according to NP_002688.2.
Epitope
Internal region (near N Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-POU2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-POU2F1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-POU2F1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-POU2F1 antibody
(0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-POU2F1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for POU2F1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,082 Da
NCBI Official Full Name
POU domain, class 2, transcription factor 1
NCBI Official Synonym Full Names
POU class 2 homeobox 1
NCBI Official Symbol
POU2F1  [Similar Products]
NCBI Official Synonym Symbols
OCT1; OTF1; oct-1B
  [Similar Products]
NCBI Protein Information
POU domain, class 2, transcription factor 1
UniProt Protein Name
POU domain, class 2, transcription factor 1
UniProt Synonym Protein Names
NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
UniProt Gene Name
POU2F1  [Similar Products]
UniProt Synonym Gene Names
OCT1; OTF1; Oct-1; OTF-1  [Similar Products]
UniProt Entry Name
PO2F1_HUMAN
NCBI Summary for POU2F1
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
UniProt Comments for POU2F1
Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: negative regulation of transcription, DNA-dependent; snRNA transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000