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anti-SYK antibody :: Goat Spleen tyrosine kinase/SYK Polyclonal Antibody

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Catalog # MBS421218
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Spleen tyrosine kinase/SYK, Polyclonal Antibody

Also Known As

Goat anti-Spleen tyrosine kinase/SYK Antibody

Product Synonym Names
SYK; spleen tyrosine kinase; HGNC: 11491; DKFZp313N1010; FLJ25043; FLJ37489; OTTHUMP00000021628; OTTHUMP00000021629; SYK antibody; spleen tyrosine kinase antibody; HGNC: 11491 antibody; DKFZp313N1010 antibody; FLJ25043 antibody; FLJ37489 antibody; OTTHUMP00000021628 antibody; OTTHUMP00000021629 antibody; Spleen tyrosine kinase; Spleen tyrosine kinase / SYK
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
635
Immunogen Sequence
KYLQQNRHVK DKN
OMIM
600085
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KYLQQNRHVKDKN, from the internal region of the protein sequence according to NP_003168.2; NP_001128524.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_003168.2; NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1; NP_001128524.1 and NP_001167639.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SYK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SYK antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SYK antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 70kDa band observed in Human Bone marrow, Lymph Node, and Spleen lysates (calculated MW of 72.1kDa according to NP_003168.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-SYK antibody
(1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SYK antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SYK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001128524.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
69,510 Da
NCBI Official Full Name
tyrosine-protein kinase SYK isoform Syk(L)
NCBI Official Synonym Full Names
spleen tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
p72-Syk
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NCBI Protein Information
tyrosine-protein kinase SYK
UniProt Protein Name
Tyrosine-protein kinase SYK
UniProt Synonym Protein Names
Spleen tyrosine kinase; p72-Syk
Protein Family
UniProt Gene Name
UniProt Entry Name
KSYK_HUMAN
NCBI Summary for SYK
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for SYK
Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: B cell receptor complex; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; nucleus; plasma membrane; protein complex; T cell receptor complex

Molecular Function: ATP binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity

Biological Process: activation of JNK activity; adaptive immune response; angiogenesis; B cell receptor signaling pathway; beta selection; blood vessel morphogenesis; cell proliferation; defense response to bacterium; inflammatory response; innate immune response; integrin-mediated signaling pathway; leukocyte activation during immune response; leukocyte adhesion; leukotriene biosynthetic process; lymph vessel development; macrophage activation during immune response; neutrophil activation during immune response; neutrophil chemotaxis; organ morphogenesis; peptidyl-serine phosphorylation; platelet activation; positive regulation of alpha-beta T cell differentiation; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell differentiation; positive regulation of bone resorption; positive regulation of calcium-mediated signaling; positive regulation of cell adhesion mediated by integrin; positive regulation of cytokine secretion; positive regulation of gamma-delta T cell differentiation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interferon type I production; positive regulation of interleukin-3 biosynthetic process; positive regulation of mast cell degranulation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of receptor internalization; protein amino acid phosphorylation; receptor internalization; regulation of neutrophil degranulation; regulation of phagocytosis; regulation of superoxide release; regulation of transcription factor activity; serotonin secretion by platelet; stimulatory C-type lectin receptor signaling pathway; transcription factor import into nucleus; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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