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anti-TFEB antibody :: Goat TFEB Polyclonal Antibody

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Catalog # MBS420811
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

TFEB, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-TFEB (N Terminus) Antibody

Product Synonym Names
Goat Anti-TFEB (internal) Antibody; TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB; RP4-696P19.3; AlphaTFEB; TCFEB; bHLHe35; OTTHUMP00000043540; TFEB antibody; transcription factor EB antibody; TCFEB antibody; T-cell transcription factor EB antibody; RP4-696P19.3 antibody; AlphaTFEB antibody; TCFEB antibody; bHLHe35 antibody; OTTHUMP00000043540 antibody; TFEB (N Terminus)
Antibody/Peptide Pairs
TFEB peptide (MBS428090) is used for blocking the activity of TFEB antibody (MBS420811)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600744
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human;
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen
Peptide with sequence ASRIGLRMQLMRE-C, from the N Terminus of the protein sequence according to NP_009093.1.NP_001161299.2; NP_001258872.1
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-TFEB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TFEB antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-TFEB antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.

Western Blot: Aprox.55kDa band observed in Human Thymus and in lysates of cell line Jurkat and approx. 60 kDa in lysates of cell line HeLa, (calculated MW of 52.9 kDa according to NP_009093.1 and 54.2 kDa according to NP_001161299.2)Recommended concentration:0.3-1 ug/mL.Primary incubation 1 hour at room temperature.

Chromatin Immunoprecipitation: This antibody was confirmed in ChIP on SK-MEL-5 cells. This antibody was obtained from a previous batch (different goat).

Testing Data TD of anti-TFEB antibody
MBS420811(0.5 ug/mL)staining of HeLa (A) and(0.3ug/mL) Jurkat(B)Cell lysate(35 ug protein in RIPA buffer).Detected by chemiluminescence.
anti-TFEB antibody Testing Data TD image
Testing Data TD of anti-TFEB antibody
MBS420811 (1.0 ug/mL) staining of Human Thymus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
anti-TFEB antibody Testing Data TD image
NCBI/Uniprot data below describe general gene information for TFEB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
transcription factor EB isoform 1
NCBI Official Synonym Full Names
transcription factor EB
NCBI Official Symbol
NCBI Official Synonym Symbols
TCFEB; BHLHE35; ALPHATFEB
  [Similar Products]
NCBI Protein Information
transcription factor EB
UniProt Protein Name
Transcription factor EB
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 35; bHLHe35
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BHLHE35; bHLHe35  [Similar Products]
UniProt Entry Name
TFEB_HUMAN
UniProt Comments for TFEB
TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: adaptive immune response; autophagy; embryonic placenta development; humoral immune response; lysosome organization and biogenesis; positive regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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