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anti-UCHL1 antibody :: Goat UCHL1 Polyclonal Antibody

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Catalog # MBS422428
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

UCHL1, Polyclonal Antibody

Also Known As

Goat anti-UCHL1 (aa 211-221) Antibody

Product Synonym Names
Goat Anti-UCHL1 (aa 58-68) Antibody; UCHL1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); PARK5; PGP9.5; PGP95; Uch-L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin carboxyl-terminal esterase L1; neuron cytoplasmic protein 9.5 antibody; PARK5 antibody; PGP9.5 antibody; PGP95 antibody; ubiquitin carboxyl-terminal esterase L1 antibody; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) antibody; ubiquitin C-terminal hydrolase antibody; Uch-L1 antibody; UCHL1 antibody; UCHL1 (aa 211-221)
Antibody/Peptide Pairs
UCHL1 peptide (MBS427374) is used for blocking the activity of UCHL1 antibody (MBS422428)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
223
Immunogen Sequence
EVRFSAVALC K
OMIM
191342
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence EVRFSAVALCK, from the C Terminus of the protein sequence according to NP_004172.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-UCHL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-UCHL1 antibody
Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
Application Notes for anti-UCHL1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 26kDa band observed in Human Brain (Hippocampus) and Mouse and Rat Brain lysates (calculated MW of 24.8kDa according to Human NP_004172.2, Mouse NP_035800.2 and Rat NP_058933.2). Recommended concentration: 0.01-0.03ug/ml.

Western Blot (WB) of anti-UCHL1 antibody
(0. 01ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-UCHL1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for UCHL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,824 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
UCHL1  [Similar Products]
NCBI Official Synonym Symbols
NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
  [Similar Products]
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
UniProt Gene Name
UCHL1  [Similar Products]
UniProt Synonym Gene Names
UCH-L1; PGP9.5  [Similar Products]
UniProt Entry Name
UCHL1_HUMAN
NCBI Summary for UCHL1
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
UniProt Comments for UCHL1
UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Protease; Ubiquitin conjugating system; Ligase; EC 3.4.19.12; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum membrane; myelin sheath; nucleoplasm; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: adult walking behavior; axon target recognition; axon transport of mitochondrion; cell proliferation; eating behavior; muscle fiber development; negative regulation of MAP kinase activity; neuromuscular process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of macroautophagy; sensory perception of pain

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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