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anti-HSD17B10 antibody :: Goat ABAD/HADH2 Polyclonal Antibody

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Catalog # MBS421448
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ABAD/HADH2 (HSD17B10), Polyclonal Antibody

Also Known As

Goat anti-ABAD/HADH2 Antibody

Product Synonym Names
HSD17B10; HADH2; ABAD; ERAB; MHBD; 17b-HSD10; hydroxyacyl-Coenzyme A dehydrogenase, type II; type 10 17b-HSD; AB-binding alcohol dehydrogenase; type 10 17beta-hydroxysteroid dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 10; RP3-339A18.2; 17b-HSD10; HADH2 antibody; ABAD antibody; ERAB antibody; MHBD antibody; HSD17B10 antibody; 17b-HSD10 antibody; hydroxyacyl-Coenzyme A dehydrogenase; type II antibody; type 10 17b-HSD antibody; AB-binding alcohol dehydrogenase antibody; type 10 17beta-hydroxysteroid dehydrogenase antibody; ABAD / HADH2
Product Synonym Gene Name
ABAD; HADH2[Similar Products]
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
261
Immunogen Sequence
CIRLDGAIRM QP
OMIM
300220
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CIRLDGAIRMQP, from the C Terminus of the protein sequence according to NP_004484.1; NP_001032900.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-HSD17B10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HSD17B10 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSD17B10 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2ug/ml. A minor band of unknown identity was also consistently observed at appr 38kDa. This band was successfully blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-HSD17B10 antibody
Staining (0.3ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-HSD17B10 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HSD17B10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001032900.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,984 Da
NCBI Official Full Name
3-hydroxyacyl-CoA dehydrogenase type-2 isoform 1
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 10
NCBI Official Symbol
HSD17B10  [Similar Products]
NCBI Official Synonym Symbols
ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
  [Similar Products]
NCBI Protein Information
3-hydroxyacyl-CoA dehydrogenase type-2
UniProt Protein Name
3-hydroxyacyl-CoA dehydrogenase type-2
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 10 (EC:1.1.1.51); 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC:1.1.1.178); 3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Short chain dehydrogenase/reductase family 5C member 1; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH
UniProt Gene Name
HSD17B10  [Similar Products]
UniProt Synonym Gene Names
ERAB; HADH2; MRPP2; SCHAD; SDR5C1; XH98G2; 17-beta-HSD 10; Mitochondrial RNase P protein 2  [Similar Products]
UniProt Entry Name
HCD2_HUMAN
NCBI Summary for HSD17B10
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]
UniProt Comments for HSD17B10
HADH2: Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). Defects in HSD17B10 are the cause of 2-methyl-3- hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency). MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills. Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10). MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior. A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17); also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub- average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X- linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non- syndromic X-linked mental retardation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Mitochondrial; EC 1.1.1.178; EC 1.1.1.51; EC 1.1.1.35

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion; plasma membrane

Molecular Function: 3(or 17)beta-hydroxysteroid dehydrogenase activity; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity; 3-hydroxyacyl-CoA dehydrogenase activity; 7-alpha-hydroxysteroid dehydrogenase activity; acetoacetyl-CoA reductase activity; beta-amyloid binding; estradiol 17-beta-dehydrogenase activity; estrogen receptor binding; identical protein binding; NAD binding; protein binding; steroid binding

Biological Process: branched chain family amino acid catabolic process; cell aging; Leydig cell differentiation; lipid metabolic process; protein homotetramerization

Disease: 17-beta-hydroxysteroid Dehydrogenase X Deficiency; Mental Retardation, X-linked, Syndromic 10
Product References and Citations for anti-HSD17B10 antibody
Tilton RG, Haidacher SJ, Lejeune WS, Zhang X, Zhao Y, Kurosky A, Brasier AR, Denner L.; Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry.; Proteomics.2007 May;7(10): 1729-42.; Pubmed 17436268

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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