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anti-SH3GLB1 antibody :: Goat BIF-1/SH3GLB1 Polyclonal Antibody

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Catalog # MBS420183
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

BIF-1/SH3GLB1, Polyclonal Antibody

Also Known As

Goat anti-BIF-1/SH3GLB1 Antibody

Product Synonym Names
SH3GLB1; Bif-1; SH3-domain GRB2-like endophilin B1; CGI-61; KIAA0491; endophilin B1; Bax-interacting factor 1; SH3-containing protein SH3GLB1; SH3-domain, GRB2-like, endophilin B1; SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1; dJ612B15.2; SH3GLB1 antibody; Bif-1 antibody; SH3-domain GRB2-like endophilin B1 antibody; CGI-61 antibody; KIAA0491 antibody; endophilin B1 antibody; Bax-interacting factor 1 antibody; SH3-containing protein SH3GLB1 antibody; SH3-domain; GRB2-like; endophilin B1 antibody; SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1 antibody; dJ612B15.2 antibody; BIF-1 / SH3GLB1
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
365
Immunogen Sequence
QKGKVPITYL ELLN
OMIM
609287
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SH3GLB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SH3GLB1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SH3GLB1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1. 0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-SH3GLB1 antibody
Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-SH3GLB1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SH3GLB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,318 Da
NCBI Official Full Name
endophilin-B1 isoform 1
NCBI Official Synonym Full Names
SH3 domain containing GRB2 like endophilin B1
NCBI Official Symbol
SH3GLB1  [Similar Products]
NCBI Official Synonym Symbols
Bif-1; CGI-61; PPP1R70; dJ612B15.2
  [Similar Products]
NCBI Protein Information
endophilin-B1
UniProt Protein Name
Endophilin-B1
UniProt Synonym Protein Names
Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1
Protein Family
UniProt Gene Name
SH3GLB1  [Similar Products]
UniProt Synonym Gene Names
KIAA0491; Bif-1  [Similar Products]
UniProt Entry Name
SHLB1_HUMAN
NCBI Summary for SH3GLB1
This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
UniProt Comments for SH3GLB1
SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Mitochondrial; Transferase; EC 2.3.1.-; Apoptosis

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytoplasmic vesicle; Golgi membrane; midbody; mitochondrial outer membrane

Molecular Function: identical protein binding; lipid binding; protein binding; protein homodimerization activity

Biological Process: apoptosis; autophagic cell death; autophagy; cellular response to amino acid starvation; cellular response to glucose starvation; positive regulation of autophagy; positive regulation of protein oligomerization; protein oligomerization; receptor catabolic process; regulation of cytokinesis; regulation of macroautophagy; regulation of protein stability
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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