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anti-CD2BP2 antibody :: Goat CD2BP2 Polyclonal Antibody

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Catalog # MBS420728
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

CD2BP2, Polyclonal Antibody

Full Product Name

Goat anti-CD2BP2 (N Terminus) Antibody

Product Synonym Names
CD2BP2; FWP010; LIN1; Snu40; CD2 antigen (cytoplasmic tail) binding protein 2; CD2 (cytoplasmic tail) binding protein 2; CD2 antigen (cytoplasmic tail)-binding protein 2; U5-52K; CD2 binding protein 2; CD2 cytoplasmic domain binding protein 2; U5 snRNP 52; CD2BP2 antibody; FWP010 antibody; LIN1 antibody; Snu40 antibody; CD2 antigen (cytoplasmic tail) binding protein 2 antibody; CD2 (cytoplasmic tail) binding protein 2 antibody; CD2 antigen (cytoplasmic tail)-binding protein 2 antibody; U5-52K antibody; CD2 binding protein 2 antibody; CD2 cytoplasmic domain binding protein 2 antibody; U5 snRNP 52K protein antibody; CD2BP2 (N Terminus)
Antibody/Peptide Pairs
CD2BP2 peptide (MBS425252) is used for blocking the activity of CD2BP2 antibody (MBS420728)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
PKRKVTFQGV GDE
OMIM
604470
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence PKRKVTFQGVGDE-C, from the N Terminus of the protein sequence according to NP_006101.1.
Epitope
N Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CD2BP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD2BP2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CD2BP2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in select splenocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 50kDa band observed in Human Lymph Node, Thymus and Tonsil lysates (calculated MW of 37.6kDa according to NP_006101.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nishizawa et al, Proc Natl Acad Sci U S A.1998 Dec 8;95(25): 14897-902). Recommended concentration: 0.3-1. 0ug/ml.

Western Blot (WB) of anti-CD2BP2 antibody
(0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CD2BP2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CD2BP2 antibody
(5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-CD2BP2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD2BP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37,646 Da
NCBI Official Full Name
CD2 antigen cytoplasmic tail-binding protein 2
NCBI Official Synonym Full Names
CD2 (cytoplasmic tail) binding protein 2
NCBI Official Symbol
CD2BP2  [Similar Products]
NCBI Official Synonym Symbols
LIN1; Snu40; FWP010; U5-52K; PPP1R59
  [Similar Products]
NCBI Protein Information
CD2 antigen cytoplasmic tail-binding protein 2
UniProt Protein Name
CD2 antigen cytoplasmic tail-binding protein 2
UniProt Synonym Protein Names
U5 snRNP 52K protein; U5-52K
UniProt Gene Name
CD2BP2  [Similar Products]
UniProt Synonym Gene Names
KIAA1178; CD2 cytoplasmic domain-binding protein 2; CD2 tail-binding protein 2; U5-52K  [Similar Products]
UniProt Entry Name
CD2B2_HUMAN
NCBI Summary for CD2BP2
This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined. [provided by RefSeq, Nov 2008]
UniProt Comments for CD2BP2
CD2BP2: Binds the cytoplasmic domain of CD2 through the GYF domain.

Protein type: Adaptor/scaffold; Spliceosome; RNA processing

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasm; nuclear speck; nucleoplasm; nucleus; snRNP U5; U4/U6 x U5 tri-snRNP complex

Molecular Function: protein binding; ribonucleoprotein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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