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anti-DUSP10 antibody :: Goat DUSP10/MKP5 Polyclonal Antibody

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Catalog # MBS420639
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

DUSP10/MKP5, Polyclonal Antibody

Also Known As

Goat anti-DUSP10/MKP5 Antibody

Product Synonym Names
DUSP10; MKP5; dual specificity phosphatase 10; MKP-5; dual specificity phosphatase MKP-5; serine/threonine specific protein phosphatase; DUSP10 antibody; MKP5 antibody; dual specificity phosphatase 10 antibody; MKP-5 antibody; dual specificity phosphatase MKP-5 antibody; serine/threonine specific protein phosphatase antibody; DUSP10 / MKP5
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
482
Immunogen Sequence
CRILTPKLMG VETVV
OMIM
608867
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CRILTPKLMGVETVV, from the C Terminus of the protein sequence according to NP_009138.1; NP_653329.1; NP_653330.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both reported Human isofoms. The variants NP_653329.1 and NP_653330.1 represent identical protein.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-DUSP10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-DUSP10 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-DUSP10 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 53kDa band seen in Human Liver lysate. Recommended for use at 0.5-2ug/ml.

Western Blot (WB) of anti-DUSP10 antibody
Staining (0.5ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-DUSP10 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DUSP10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_653329.1; NP_653330.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,110 Da
NCBI Official Full Name
dual specificity protein phosphatase 10
NCBI Official Synonym Full Names
dual specificity phosphatase 10
NCBI Official Symbol
DUSP10  [Similar Products]
NCBI Official Synonym Symbols
MKP5; MKP-5
  [Similar Products]
NCBI Protein Information
dual specificity protein phosphatase 10
UniProt Protein Name
Dual specificity protein phosphatase 10
UniProt Synonym Protein Names
Mitogen-activated protein kinase phosphatase 5; MAP kinase phosphatase 5; MKP-5
UniProt Gene Name
DUSP10  [Similar Products]
UniProt Synonym Gene Names
MKP5; MAP kinase phosphatase 5; MKP-5  [Similar Products]
UniProt Entry Name
DUS10_HUMAN
NCBI Summary for DUSP10
Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Comments for DUSP10
MKP-5: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP10 binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. Contains 1 rhodanese domain.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus

Molecular Function: MAP kinase phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity

Biological Process: inactivation of MAPK activity; JNK cascade; negative regulation of JNK activity; negative regulation of oligodendrocyte differentiation; oligodendrocyte differentiation; protein amino acid dephosphorylation; regulation of adaptive immune response; response to lipopolysaccharide; response to stress
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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