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anti-MID1IP1 antibody :: Goat MIG12 Polyclonal Antibody

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Catalog # MBS422493
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

MIG12 (MID1IP1), Polyclonal Antibody

Full Product Name

Goat anti-MIG12 Antibody

Product Synonym Names
MID1IP1; MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)); FLJ10386; G12-like; MIG12; STRAIT11499; THRSPL; MID1 interacting G12-like protein; MID1 interacting protein 1 (gastrulation specific G12-like); FLJ10386 antibody; G12-like antibody; MID1 interacting G12-like protein antibody; MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)) antibody; MID1 interacting protein 1 (gastrulation specific G12-like) antibody; MIG12 antibody; STRAIT11499 antibody; THRSPL antibody; MID1IP1 antibody
Product Synonym Gene Name
Antibody/Peptide Pairs
MIG12 peptide (MBS427600) is used for blocking the activity of MIG12 antibody (MBS422493)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
HTVLSKLTRK
OMIM
300961
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-HTVLSKLTRK, from the internal region of the protein sequence according to NP_067065.1.
Epitope
Internal region
Note
Reported variants represent identical protein: NP_001092261.1, NP_067065.1, NP_001092260.1
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-MID1IP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MID1IP1 antibody
Peptide ELISA (EIA), Western Blot (WB-Trf)
Application Notes for anti-MID1IP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human MID1IP1 (myc and DYKDDDDK tagged), a band of approx.28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.

Western Blot (WB) of anti-MID1IP1 antibody
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1IP1 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with
anti-MID1IP1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MID1IP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,202 Da
NCBI Official Full Name
mid1-interacting protein 1
NCBI Official Synonym Full Names
MID1 interacting protein 1
NCBI Official Symbol
MID1IP1  [Similar Products]
NCBI Official Synonym Symbols
S14R; MIG12; THRSPL; G12-like; STRAIT11499
  [Similar Products]
NCBI Protein Information
mid1-interacting protein 1
UniProt Protein Name
Mid1-interacting protein 1
UniProt Synonym Protein Names
Gastrulation-specific G12-like protein; Mid1-interacting G12-like protein; Protein STRAIT11499; Spot 14-related protein; S14R; Spot 14-R
Protein Family
UniProt Gene Name
MID1IP1  [Similar Products]
UniProt Synonym Gene Names
MIG12; S14R; Spot 14-R  [Similar Products]
UniProt Entry Name
M1IP1_HUMAN
UniProt Comments for MID1IP1
MID1IP1: Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules. Belongs to the SPOT14 family.

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: cytosol; microtubule; microtubule cytoskeleton; nucleus

Molecular Function: protein binding; protein C-terminus binding

Biological Process: lipid metabolic process; negative regulation of microtubule depolymerization; positive regulation of fatty acid biosynthetic process; positive regulation of ligase activity; protein polymerization; regulation of lipid biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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