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anti-NCAM2 antibody :: Goat NCAM2/OCAM Polyclonal Antibody

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Catalog # MBS421506
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

NCAM2/OCAM, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-NCAM2/OCAM Antibody

Product Synonym Names
NCAM2; neural cell adhesion molecule 2; HGNC: 7657; MGC51008; NCAM21; OCAM; NCAM2 antibody; neural cell adhesion molecule 2 antibody; HGNC: 7657 antibody; MGC51008 antibody; NCAM21 antibody; OCAM antibody; NCAM2 / OCAM
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KVSNDIIQSK EDDSK
OMIM
602040
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen
Peptide with sequence C-KVSNDIIQSKEDDSK, from the C Terminus of the protein sequence according to NP_004531.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-NCAM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NCAM2 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-NCAM2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.


Western Blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93. 0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide.


Immunohistochemistry: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration:0.5 ug/mL. Paraffin embedded Human Brain (Cortex). Recommended concentration is: 3.75ug/ml.

Immunofluorescence (IF) of anti-NCAM2 antibody
(0.5ug/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from anonymous customer.
anti-NCAM2 antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-NCAM2 antibody
(0.5ug/ml) 48h-staining of PFA-perfused cryosection of Mouse Olfactory bulb. Antigen retrieval with citrate buffer pH 6 at 95C for 10min, IF-staning for NCAM2 (red), VGLUT2 (blue) and NQO1 (green), data obtained from anonymus customer.
anti-NCAM2 antibody Immunofluorescence (IF) image
Testing Data(TD) of anti-NCAM2 antibody
(3.75 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH, AP-staining.
anti-NCAM2 antibody Testing Data(TD) image
NCBI/Uniprot data below describe general gene information for NCAM2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
neural cell adhesion molecule 2
NCBI Official Synonym Full Names
neural cell adhesion molecule 2
NCBI Official Symbol
NCAM2  [Similar Products]
NCBI Official Synonym Symbols
NCAM21
  [Similar Products]
NCBI Protein Information
neural cell adhesion molecule 2
UniProt Protein Name
Neural cell adhesion molecule 2
UniProt Gene Name
NCAM2  [Similar Products]
UniProt Synonym Gene Names
NCAM21; N-CAM-2; NCAM-2  [Similar Products]
UniProt Entry Name
NCAM2_HUMAN
NCBI Summary for NCAM2
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein and may function in selective fasciculation and zone-to-zone projection of the primary olfactory axons. [provided by RefSeq, Jul 2008]
UniProt Comments for NCAM2
NCAM2: May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: axon; integral to membrane; plasma membrane

Biological Process: neuron adhesion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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