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anti-ROBO1 antibody :: Goat ROBO1/DUTT1 Polyclonal Antibody

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Catalog # MBS421220
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ROBO1/DUTT1, Polyclonal Antibody

Full Product Name

Goat anti-ROBO1/DUTT1 (Internal) Antibody

Product Synonym Names
ROBO1; DUTT1; roundabout, axon guidance receptor, homolog 1 (Drosophila); HGNC: 10249; FLJ21882; SAX3; axon guidance receptor; roundabout 1; MGC131599; MGC133277; ROBO1 antibody; DUTT1 antibody; roundabout; axon guidance receptor; homolog 1 (Drosophila) antibody; HGNC: 10249 antibody; FLJ21882 antibody; SAX3 antibody; axon guidance receptor antibody; roundabout 1 antibody; MGC131599 antibody; MGC133277 antibody; ROBO1 / DUTT1 (Internal)
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
GDVDLSNKIN EMK
OMIM
602430
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1.
Epitope
Internal region
Note
This antibody is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ROBO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ROBO1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ROBO1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-ROBO1 antibody
(1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ROBO1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ROBO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_598334.1; NP_001139316.1; NP_001139317.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
169,661 Da
NCBI Official Full Name
roundabout homolog 1 isoform a
NCBI Official Synonym Full Names
roundabout guidance receptor 1
NCBI Official Symbol
ROBO1  [Similar Products]
NCBI Official Synonym Symbols
SAX3; DUTT1
  [Similar Products]
NCBI Protein Information
roundabout homolog 1
UniProt Protein Name
Roundabout homolog 1
UniProt Synonym Protein Names
Deleted in U twenty twenty; H-Robo-1
Protein Family
UniProt Gene Name
ROBO1  [Similar Products]
UniProt Synonym Gene Names
DUTT1  [Similar Products]
UniProt Entry Name
ROBO1_HUMAN
NCBI Summary for ROBO1
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for ROBO1
ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3p12

Cellular Component: axon; cell surface; cytoplasm; integral to plasma membrane; plasma membrane

Molecular Function: axon guidance receptor activity; identical protein binding; LRR domain binding; protein binding

Biological Process: axon guidance; axon midline choice point recognition; caspase activation; cell adhesion; cell migration during sprouting angiogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; heart development; homophilic cell adhesion; negative regulation of cell migration; negative regulation of mammary gland epithelial cell proliferation; negative regulation of negative chemotaxis; nervous system development; positive regulation of axonogenesis; positive regulation of Rho protein signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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