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anti-SPHK1 antibody :: Goat SPHK1 Polyclonal Antibody

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Catalog # MBS421620
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

SPHK1, Polyclonal Antibody

Also Known As

Goat anti-SPHK1 Antibody

Product Synonym Names
Goat Anti-SPHK1 (C-term. ) Antibody; SPHK1; sphingosine kinase 1; SPHK; SK1; SKase; SPHK1 antibody; sphingosine kinase 1 antibody; SPHK antibody; SK1 antibody; SKase antibody
Antibody/Peptide Pairs
SPHK1 peptide (MBS426444) is used for blocking the activity of SPHK1 antibody (MBS421620)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
398
Immunogen Sequence
DVDLESEKYR RLGE
OMIM
603730
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2; NP_892010.2; NP_001136073.1.
Epitope
Internal region
Note
This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SPHK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SPHK1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-SPHK1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3ug/ml.

Western Blot (WB) of anti-SPHK1 antibody
(2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SPHK1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SPHK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_892010.2; NP_001136073.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,944 Da
NCBI Official Full Name
sphingosine kinase 1 isoform 1
NCBI Official Synonym Full Names
sphingosine kinase 1
NCBI Official Symbol
SPHK1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
sphingosine kinase 1
UniProt Protein Name
Sphingosine kinase 1
UniProt Gene Name
SPHK1  [Similar Products]
UniProt Synonym Gene Names
SPHK; SPK; SK 1; SPK 1  [Similar Products]
UniProt Entry Name
SPHK1_HUMAN
NCBI Summary for SPHK1
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for SPHK1
SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; EC 2.7.1.91; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: axon; cytoplasm; cytosol; nucleus; plasma membrane; synaptic vesicle

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; magnesium ion binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity

Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cellular response to starvation; cyclooxygenase pathway; female pregnancy; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; protein folding; regulation of interleukin-1 beta production; response to amine stimulus; response to ATP; response to magnesium ion; response to progesterone stimulus; signal transduction; sphingoid catabolic process; sphingolipid biosynthetic process; sphingosine biosynthetic process; sphingosine metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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